FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2109976452> ?p ?o ?g. }

• W2109976452 endingPage "1024" @default.
• W2109976452 startingPage "1021" @default.
• W2109976452 abstract "ABSTRACT Encephalitozoon intestinalis is responsible for intestinal disease in patients with AIDS and immunocompetent patients. The infectious form is a small spore that is resistant to water treatment procedures. Its detection is very important, but detection is very cumbersome and time-consuming. Our main objective was to develop and optimize a specific flow cytometric (FC) protocol for the detection of E. intestinalis in hospital tap water and human feces. To determine the optimal specific antibody (Microspor-FA) concentration, a known concentration of E. intestinalis spores (Waterborne, Inc.) was suspended in hospital tap water and stool specimens with different concentrations of Microspor-FA, and the tap water and stool specimens were incubated under different conditions. The sensitivity limit and specificity were also evaluated. To study spore infectivity, double staining with propidium iodide (PI) and Microspor-FA was undertaken. Distinct approaches for filtration and centrifugation of the stool specimens were used. E. intestinalis spores stained with 10 μg/ml of Microspor-FA at 25°C overnight provided the best results. The detection limit was 5 × 10 4 spores/ml, and good specificity was demonstrated. Simultaneous staining with Microspor-FA and PI ensured that the E. intestinalis spores were dead and therefore noninfectious. With the stool specimens, better spore recovery was observed with a saturated solution of NaCl and centrifugation at 1,500 × g for 15 min. A new approach for the detection of E. intestinalis from tap water or human feces that ensures that the spores are not viable is now available and represents an important step for the prevention of this threat to public health." @default.
• W2109976452 created "2016-06-24" @default.
• W2109976452 creator A5026791846 @default.
• W2109976452 creator A5049493833 @default.
• W2109976452 creator A5077799127 @default.
• W2109976452 date "2009-07-01" @default.
• W2109976452 modified "2023-10-16" @default.
• W2109976452 title "Cytometric Approach for Detection of <i>Encephalitozoon intestinalis</i> , an Emergent Agent" @default.
• W2109976452 cites W12146774 @default.
• W2109976452 cites W1634682499 @default.
• W2109976452 cites W1801770934 @default.
• W2109976452 cites W1827553253 @default.
• W2109976452 cites W2001748299 @default.
• W2109976452 cites W2019409308 @default.
• W2109976452 cites W2021672917 @default.
• W2109976452 cites W2046048905 @default.
• W2109976452 cites W2097105412 @default.
• W2109976452 cites W2106276379 @default.
• W2109976452 cites W2106794780 @default.
• W2109976452 cites W2107062050 @default.
• W2109976452 cites W2112111732 @default.
• W2109976452 cites W2120937665 @default.
• W2109976452 cites W2134092786 @default.
• W2109976452 cites W2144786006 @default.
• W2109976452 cites W2145598566 @default.
• W2109976452 cites W2155541542 @default.
• W2109976452 cites W2159420465 @default.
• W2109976452 cites W2159799484 @default.
• W2109976452 cites W2163603817 @default.
• W2109976452 cites W2319468699 @default.
• W2109976452 doi "https://doi.org/10.1128/cvi.00031-09" @default.
• W2109976452 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2708408" @default.
• W2109976452 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19439525" @default.
• W2109976452 hasPublicationYear "2009" @default.
• W2109976452 type Work @default.
• W2109976452 sameAs 2109976452 @default.
• W2109976452 citedByCount "12" @default.
• W2109976452 countsByYear W21099764522012 @default.
• W2109976452 countsByYear W21099764522013 @default.
• W2109976452 countsByYear W21099764522014 @default.
• W2109976452 countsByYear W21099764522017 @default.
• W2109976452 countsByYear W21099764522018 @default.
• W2109976452 crossrefType "journal-article" @default.
• W2109976452 hasAuthorship W2109976452A5026791846 @default.
• W2109976452 hasAuthorship W2109976452A5049493833 @default.
• W2109976452 hasAuthorship W2109976452A5077799127 @default.
• W2109976452 hasBestOaLocation W21099764522 @default.
• W2109976452 hasConcept C106358424 @default.
• W2109976452 hasConcept C119128265 @default.
• W2109976452 hasConcept C127413603 @default.
• W2109976452 hasConcept C159047783 @default.
• W2109976452 hasConcept C174618031 @default.
• W2109976452 hasConcept C17538187 @default.
• W2109976452 hasConcept C185592680 @default.
• W2109976452 hasConcept C2522874641 @default.
• W2109976452 hasConcept C2777392542 @default.
• W2109976452 hasConcept C43617362 @default.
• W2109976452 hasConcept C54355233 @default.
• W2109976452 hasConcept C55493867 @default.
• W2109976452 hasConcept C61716771 @default.
• W2109976452 hasConcept C74864618 @default.
• W2109976452 hasConcept C7623868 @default.
• W2109976452 hasConcept C86803240 @default.
• W2109976452 hasConcept C87717796 @default.
• W2109976452 hasConcept C89423630 @default.
• W2109976452 hasConceptScore W2109976452C106358424 @default.
• W2109976452 hasConceptScore W2109976452C119128265 @default.
• W2109976452 hasConceptScore W2109976452C127413603 @default.
• W2109976452 hasConceptScore W2109976452C159047783 @default.
• W2109976452 hasConceptScore W2109976452C174618031 @default.
• W2109976452 hasConceptScore W2109976452C17538187 @default.
• W2109976452 hasConceptScore W2109976452C185592680 @default.
• W2109976452 hasConceptScore W2109976452C2522874641 @default.
• W2109976452 hasConceptScore W2109976452C2777392542 @default.
• W2109976452 hasConceptScore W2109976452C43617362 @default.
• W2109976452 hasConceptScore W2109976452C54355233 @default.
• W2109976452 hasConceptScore W2109976452C55493867 @default.
• W2109976452 hasConceptScore W2109976452C61716771 @default.
• W2109976452 hasConceptScore W2109976452C74864618 @default.
• W2109976452 hasConceptScore W2109976452C7623868 @default.
• W2109976452 hasConceptScore W2109976452C86803240 @default.
• W2109976452 hasConceptScore W2109976452C87717796 @default.
• W2109976452 hasConceptScore W2109976452C89423630 @default.
• W2109976452 hasIssue "7" @default.
• W2109976452 hasLocation W21099764521 @default.
• W2109976452 hasLocation W21099764522 @default.
• W2109976452 hasLocation W21099764523 @default.
• W2109976452 hasLocation W21099764524 @default.
• W2109976452 hasOpenAccess W2109976452 @default.
• W2109976452 hasPrimaryLocation W21099764521 @default.
• W2109976452 hasRelatedWork W1932710078 @default.
• W2109976452 hasRelatedWork W1970932468 @default.
• W2109976452 hasRelatedWork W1976788601 @default.
• W2109976452 hasRelatedWork W1983916957 @default.
• W2109976452 hasRelatedWork W1991235263 @default.
• W2109976452 hasRelatedWork W1995328853 @default.
• W2109976452 hasRelatedWork W2008875895 @default.
• W2109976452 hasRelatedWork W2127902400 @default.

• next