Matches in SemOpenAlex for { <https://semopenalex.org/work/W2110054463> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2110054463 endingPage "1291" @default.
- W2110054463 startingPage "1283" @default.
- W2110054463 abstract "Plant aspartate aminotransferase (AAT, EC 2.6.1.1) plays a key role in primary nitrogen assimilation, the transfer of reducing equivalents and the interchanges of carbon and nitrogen pools between subcellular compartments. We investigated the AAT family in conifers using maritime pine as the experimental model. Genes for cytosolic, mitochondrial and two plastidic isoenzymes (eukaryotic- and prokaryotic-types) were identified and their deduced amino acid sequences compared. The primary structure of the eukaryotic-type enzymes is quite well conserved, whereas the prokaryotic-type AAT is highly divergent (15% of identity). These molecular data were confirmed by the absence of immunological cross-reactivity between the two types of native AATs. The mature prokaryotic-type polypeptide was overexpressed in Escherichia coli, and the native enzyme was purified to apparent homogeneity and its molecular properties determined. The fully active recombinant holoenzyme showed highest catalytic activity at 50–60 °C and was moderately thermostable, retaining about 50% of its activity after incubation at 70 °C for 5–10 min. The presence of pyridoxal 5′-phosphate significantly increased the thermostability of the enzyme. These molecular characteristics were exploited to develop a rapid protocol for the purification of this prokaryotic-type enzyme from pine cotyledons. The results will be useful for studying aspartate and amino acid metabolism in trees." @default.
- W2110054463 created "2016-06-24" @default.
- W2110054463 creator A5003508045 @default.
- W2110054463 creator A5012382468 @default.
- W2110054463 creator A5036006902 @default.
- W2110054463 date "2007-09-01" @default.
- W2110054463 modified "2023-10-03" @default.
- W2110054463 title "The aspartate aminotransferase family in conifers: biochemical analysis of a prokaryotic-type enzyme from maritime pine" @default.
- W2110054463 cites W1773196754 @default.
- W2110054463 cites W1951749689 @default.
- W2110054463 cites W1968195251 @default.
- W2110054463 cites W1991770108 @default.
- W2110054463 cites W2015896257 @default.
- W2110054463 cites W2024074230 @default.
- W2110054463 cites W2024693213 @default.
- W2110054463 cites W2029949059 @default.
- W2110054463 cites W2035827123 @default.
- W2110054463 cites W2044057243 @default.
- W2110054463 cites W2053577577 @default.
- W2110054463 cites W2055043387 @default.
- W2110054463 cites W2063703192 @default.
- W2110054463 cites W2068398239 @default.
- W2110054463 cites W2078594804 @default.
- W2110054463 cites W2078737319 @default.
- W2110054463 cites W2084215554 @default.
- W2110054463 cites W2099206268 @default.
- W2110054463 cites W2106882534 @default.
- W2110054463 cites W2128635872 @default.
- W2110054463 cites W2131044474 @default.
- W2110054463 cites W2133786190 @default.
- W2110054463 cites W2135984795 @default.
- W2110054463 cites W2137776930 @default.
- W2110054463 cites W2151733864 @default.
- W2110054463 cites W2158509746 @default.
- W2110054463 cites W2158534602 @default.
- W2110054463 cites W21916439 @default.
- W2110054463 cites W3023032660 @default.
- W2110054463 doi "https://doi.org/10.1093/treephys/27.9.1283" @default.
- W2110054463 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17545128" @default.
- W2110054463 hasPublicationYear "2007" @default.
- W2110054463 type Work @default.
- W2110054463 sameAs 2110054463 @default.
- W2110054463 citedByCount "15" @default.
- W2110054463 countsByYear W21100544632013 @default.
- W2110054463 countsByYear W21100544632014 @default.
- W2110054463 countsByYear W21100544632015 @default.
- W2110054463 countsByYear W21100544632017 @default.
- W2110054463 countsByYear W21100544632019 @default.
- W2110054463 countsByYear W21100544632020 @default.
- W2110054463 countsByYear W21100544632021 @default.
- W2110054463 crossrefType "journal-article" @default.
- W2110054463 hasAuthorship W2110054463A5003508045 @default.
- W2110054463 hasAuthorship W2110054463A5012382468 @default.
- W2110054463 hasAuthorship W2110054463A5036006902 @default.
- W2110054463 hasConcept C104317684 @default.
- W2110054463 hasConcept C119795356 @default.
- W2110054463 hasConcept C181199279 @default.
- W2110054463 hasConcept C2781216036 @default.
- W2110054463 hasConcept C3119751 @default.
- W2110054463 hasConcept C515207424 @default.
- W2110054463 hasConcept C547475151 @default.
- W2110054463 hasConcept C55493867 @default.
- W2110054463 hasConcept C86803240 @default.
- W2110054463 hasConcept C98539663 @default.
- W2110054463 hasConceptScore W2110054463C104317684 @default.
- W2110054463 hasConceptScore W2110054463C119795356 @default.
- W2110054463 hasConceptScore W2110054463C181199279 @default.
- W2110054463 hasConceptScore W2110054463C2781216036 @default.
- W2110054463 hasConceptScore W2110054463C3119751 @default.
- W2110054463 hasConceptScore W2110054463C515207424 @default.
- W2110054463 hasConceptScore W2110054463C547475151 @default.
- W2110054463 hasConceptScore W2110054463C55493867 @default.
- W2110054463 hasConceptScore W2110054463C86803240 @default.
- W2110054463 hasConceptScore W2110054463C98539663 @default.
- W2110054463 hasIssue "9" @default.
- W2110054463 hasLocation W21100544631 @default.
- W2110054463 hasLocation W21100544632 @default.
- W2110054463 hasOpenAccess W2110054463 @default.
- W2110054463 hasPrimaryLocation W21100544631 @default.
- W2110054463 hasRelatedWork W1763215782 @default.
- W2110054463 hasRelatedWork W1986393148 @default.
- W2110054463 hasRelatedWork W1997589721 @default.
- W2110054463 hasRelatedWork W2009813704 @default.
- W2110054463 hasRelatedWork W2037443437 @default.
- W2110054463 hasRelatedWork W2057601574 @default.
- W2110054463 hasRelatedWork W2060038873 @default.
- W2110054463 hasRelatedWork W2110054463 @default.
- W2110054463 hasRelatedWork W2150377117 @default.
- W2110054463 hasRelatedWork W2475169532 @default.
- W2110054463 hasVolume "27" @default.
- W2110054463 isParatext "false" @default.
- W2110054463 isRetracted "false" @default.
- W2110054463 magId "2110054463" @default.
- W2110054463 workType "article" @default.