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• W2110103522 abstract "In vitro somatic embryogenesis was studied in composite maize cultivar, Gaurav by culturing imma- ture embryos. To obtain efficient callus, immature em- bryos were inoculated in MS medium supplemented with 5 mgl -1 2,4-D and 2 mgl -1 NAA + 1 mgl -1 BAP under 16 hr photoperiod and 150 µmolm -2 s -1 light intensity at 28±2°C. Higher percentage of callus formation was reported in media containing 2,4-D. Longer duration of subculturing resulted in browning of calli and degradation of embry- oids. The overall frequency of embryogenesis ranged from 65-78% and frequency of green shoot formation ranged from 35-48%. In order to analyze the interactive effect of polyamines and GA3 on somatic embryogenesis, the proliferated calli were inoculated in MS medium sup- plemented with twenty seven combinations of polyamines (Putrescine, Spermine and Spermidine) and GA3. Higher concentration (150 µM) of Putrescine with GA3 (50-150 µM) was found to be most effective for in- ducing somatic embryogenesis. Though, spermine at low- er concentration (50 µM) with GA3 showed the drastic in- crease but putrescine at higher concentration was found comparatively more effective. Spermidine at varying con- centrations (50-150 µM) gave poor results only in combi- nation with lower concentration (50 µM) of GA3. It can be concluded that spermine at lower concentration (50µM), while putrescine only at higher concentration (150 µM) exhibited positive interaction with GA3 in terms of somat- ic embryogenesis. Present findings may be used as an ef- fective tool for advancing genetic purity of hybrids and composite maize cultivars production in future and also for studying the physiological and molecular aspects of genetic transformation." @default.
• W2110103522 created "2016-06-24" @default.
• W2110103522 creator A5006447932 @default.
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• W2110103522 date "2010-01-01" @default.
• W2110103522 modified "2023-09-23" @default.
• W2110103522 title "Interactive effect of GA3 and polyamines on in vitro somatic embryogenesis from immature embryos in maize (Zea mays L.)." @default.
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