Matches in SemOpenAlex for { <https://semopenalex.org/work/W2110145608> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2110145608 endingPage "635" @default.
- W2110145608 startingPage "629" @default.
- W2110145608 abstract "Endotoxin, also known as lipopolysaccharide (LPS), is the major mediator of septic shock due to Gram‐negative bacterial infection. Chemically synthesized S3 peptide, derived from Sushi3 domain of Factor C, which is the endotoxin‐sensitive serine protease of the limulus coagulation cascade, was previously shown to bind and neutralize LPS activity. For large‐scale production of this peptide and to mimick other pathogen‐recognizing molecules, tandem multimers of the S3 gene were constructed and expressed in Escherichia coli. The recombinant tetramer of S3 (rS3‐4mer) was purified by anion exchange and digested into monomers (rS3‐1mer). Both the rS3‐4mer and rS3‐1mer were functionally analyzed for their ability to bind LPS by an ELISA‐based method and surface plasmon resonance. The LAL inhibition and TNFα‐release test showed that rS3‐1 mer can neutralize the LPS activity as effectively as the synthetic S3 peptide, while rS3‐4mer displays an enhanced inhibitory effect on LPS‐induced activities. Both recombinant peptides exhibited low cytotoxicity and no haemolytic activity on human cells. This evidence suggests that the recombinant sushi peptides have potential use for the detection, removal of endotoxin and/or anti‐endotoxin strategies." @default.
- W2110145608 created "2016-06-24" @default.
- W2110145608 creator A5016756488 @default.
- W2110145608 creator A5017134695 @default.
- W2110145608 creator A5043859457 @default.
- W2110145608 creator A5062156792 @default.
- W2110145608 creator A5081012134 @default.
- W2110145608 date "2003-08-01" @default.
- W2110145608 modified "2023-09-23" @default.
- W2110145608 title "Tandem repeats of Sushi3 peptide with enhanced LPS-binding and -neutralizing activities" @default.
- W2110145608 cites W129301507 @default.
- W2110145608 cites W1527888204 @default.
- W2110145608 cites W1673257055 @default.
- W2110145608 cites W1937514773 @default.
- W2110145608 cites W1938224903 @default.
- W2110145608 cites W1968241763 @default.
- W2110145608 cites W1975085160 @default.
- W2110145608 cites W1989078665 @default.
- W2110145608 cites W2029852905 @default.
- W2110145608 cites W2065971267 @default.
- W2110145608 cites W2090570797 @default.
- W2110145608 cites W2096113104 @default.
- W2110145608 cites W2115406456 @default.
- W2110145608 cites W2312301886 @default.
- W2110145608 cites W2001012843 @default.
- W2110145608 doi "https://doi.org/10.1093/protein/gzg078" @default.
- W2110145608 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12968081" @default.
- W2110145608 hasPublicationYear "2003" @default.
- W2110145608 type Work @default.
- W2110145608 sameAs 2110145608 @default.
- W2110145608 citedByCount "17" @default.
- W2110145608 countsByYear W21101456082014 @default.
- W2110145608 countsByYear W21101456082017 @default.
- W2110145608 countsByYear W21101456082020 @default.
- W2110145608 countsByYear W21101456082021 @default.
- W2110145608 crossrefType "journal-article" @default.
- W2110145608 hasAuthorship W2110145608A5016756488 @default.
- W2110145608 hasAuthorship W2110145608A5017134695 @default.
- W2110145608 hasAuthorship W2110145608A5043859457 @default.
- W2110145608 hasAuthorship W2110145608A5062156792 @default.
- W2110145608 hasAuthorship W2110145608A5081012134 @default.
- W2110145608 hasBestOaLocation W21101456081 @default.
- W2110145608 hasConcept C104317684 @default.
- W2110145608 hasConcept C153911025 @default.
- W2110145608 hasConcept C181199279 @default.
- W2110145608 hasConcept C185592680 @default.
- W2110145608 hasConcept C203014093 @default.
- W2110145608 hasConcept C2776714187 @default.
- W2110145608 hasConcept C2777807008 @default.
- W2110145608 hasConcept C2778754761 @default.
- W2110145608 hasConcept C2779281246 @default.
- W2110145608 hasConcept C40767141 @default.
- W2110145608 hasConcept C547475151 @default.
- W2110145608 hasConcept C55493867 @default.
- W2110145608 hasConcept C86803240 @default.
- W2110145608 hasConceptScore W2110145608C104317684 @default.
- W2110145608 hasConceptScore W2110145608C153911025 @default.
- W2110145608 hasConceptScore W2110145608C181199279 @default.
- W2110145608 hasConceptScore W2110145608C185592680 @default.
- W2110145608 hasConceptScore W2110145608C203014093 @default.
- W2110145608 hasConceptScore W2110145608C2776714187 @default.
- W2110145608 hasConceptScore W2110145608C2777807008 @default.
- W2110145608 hasConceptScore W2110145608C2778754761 @default.
- W2110145608 hasConceptScore W2110145608C2779281246 @default.
- W2110145608 hasConceptScore W2110145608C40767141 @default.
- W2110145608 hasConceptScore W2110145608C547475151 @default.
- W2110145608 hasConceptScore W2110145608C55493867 @default.
- W2110145608 hasConceptScore W2110145608C86803240 @default.
- W2110145608 hasIssue "8" @default.
- W2110145608 hasLocation W21101456081 @default.
- W2110145608 hasLocation W21101456082 @default.
- W2110145608 hasOpenAccess W2110145608 @default.
- W2110145608 hasPrimaryLocation W21101456081 @default.
- W2110145608 hasRelatedWork W186976241 @default.
- W2110145608 hasRelatedWork W2016628627 @default.
- W2110145608 hasRelatedWork W2024681957 @default.
- W2110145608 hasRelatedWork W2026960302 @default.
- W2110145608 hasRelatedWork W2036951984 @default.
- W2110145608 hasRelatedWork W2045879497 @default.
- W2110145608 hasRelatedWork W2107256410 @default.
- W2110145608 hasRelatedWork W2114609271 @default.
- W2110145608 hasRelatedWork W2139138166 @default.
- W2110145608 hasRelatedWork W4255391742 @default.
- W2110145608 hasVolume "16" @default.
- W2110145608 isParatext "false" @default.
- W2110145608 isRetracted "false" @default.
- W2110145608 magId "2110145608" @default.
- W2110145608 workType "article" @default.