Matches in SemOpenAlex for { <https://semopenalex.org/work/W2110147986> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2110147986 endingPage "116" @default.
- W2110147986 startingPage "113" @default.
- W2110147986 abstract "The local lymph node assay (LLNA) is widely used to identify chemicals that are contact sensitizers. The assay involves dosing mice with the chemical on both ears and pooling the superficial parotid lymph nodes for assessment of lymphocyte proliferation as a marker of sensitization. The present study explored potential reduction in animal usage by dosing one ear with the allergen and the other with vehicle-only. The respective draining lymph nodes were processed separately for tritiated thymidine ((3)H-TdR) incorporation. Cell proliferation in proper axillary and renal nodes, as well as in the spleen was also assessed. Cross-contamination of the chemicals from the dosed ears to other parts of the body via preening was prevented by dosing restrained animals and washing off the residual chemical with saline after 4h. Dosing the left ear with 0.02% oxazolone (OX) on unrestrained animals resulted in marked cell proliferation in its draining lymph node (stimulation index, SI=12.8) and in the lymph node draining the contra-lateral vehicle-dosed ear (SI=6), as well as the proper axillary lymph nodes (SI=3.3). Increased (3)H-TdR incorporation was not observed in the renal lymph nodes (SI=1.1). Similar stimulation of cells was observed in the lymph node draining the ear contra-lateral to the 30% hexylcinnamaldehyde (HCA)-dosed ear. Increased proliferative activity was observed in contra-lateral draining lymph nodes of restrained mice demonstrating that these results cannot be attributed to cross-contamination of adjacent skin. A significant increase in proliferation of splenocytes was also observed. It is concluded that dermal application of a contact allergen, as exemplified by OX and HCA, may induce cell proliferation in the neighboring lymph nodes and spleen indicative of hapten and/or haptenated proteins diffusing through the skin to peripheral nodes and the blood to produce systemic sensitization. It is also possible that lymphatic capillaries may communicate between the left and right side of the mouse head. Thus the contra-lateral draining superficial parotid node cannot be used as a control for application of contact allergen to a single ear in a modified LLNA." @default.
- W2110147986 created "2016-06-24" @default.
- W2110147986 creator A5008160220 @default.
- W2110147986 creator A5015102283 @default.
- W2110147986 creator A5043237984 @default.
- W2110147986 creator A5078484639 @default.
- W2110147986 creator A5088401003 @default.
- W2110147986 date "2009-03-01" @default.
- W2110147986 modified "2023-09-27" @default.
- W2110147986 title "Increased cell proliferation in spleen and lymph nodes peripheral to contact allergen application site" @default.
- W2110147986 cites W1963577642 @default.
- W2110147986 cites W1989958259 @default.
- W2110147986 cites W1991952107 @default.
- W2110147986 cites W2004590673 @default.
- W2110147986 cites W2017263742 @default.
- W2110147986 cites W2033787149 @default.
- W2110147986 cites W2049481178 @default.
- W2110147986 cites W2054865262 @default.
- W2110147986 cites W2061715953 @default.
- W2110147986 cites W2131344866 @default.
- W2110147986 cites W77135042 @default.
- W2110147986 doi "https://doi.org/10.1016/j.tox.2008.12.019" @default.
- W2110147986 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19150643" @default.
- W2110147986 hasPublicationYear "2009" @default.
- W2110147986 type Work @default.
- W2110147986 sameAs 2110147986 @default.
- W2110147986 citedByCount "4" @default.
- W2110147986 countsByYear W21101479862013 @default.
- W2110147986 countsByYear W21101479862022 @default.
- W2110147986 countsByYear W21101479862023 @default.
- W2110147986 crossrefType "journal-article" @default.
- W2110147986 hasAuthorship W2110147986A5008160220 @default.
- W2110147986 hasAuthorship W2110147986A5015102283 @default.
- W2110147986 hasAuthorship W2110147986A5043237984 @default.
- W2110147986 hasAuthorship W2110147986A5078484639 @default.
- W2110147986 hasAuthorship W2110147986A5088401003 @default.
- W2110147986 hasConcept C121608353 @default.
- W2110147986 hasConcept C126322002 @default.
- W2110147986 hasConcept C142724271 @default.
- W2110147986 hasConcept C181152851 @default.
- W2110147986 hasConcept C203014093 @default.
- W2110147986 hasConcept C2776217527 @default.
- W2110147986 hasConcept C2777649267 @default.
- W2110147986 hasConcept C2777761686 @default.
- W2110147986 hasConcept C2779720271 @default.
- W2110147986 hasConcept C2779790080 @default.
- W2110147986 hasConcept C2780614586 @default.
- W2110147986 hasConcept C2780849966 @default.
- W2110147986 hasConcept C2780931953 @default.
- W2110147986 hasConcept C3018346242 @default.
- W2110147986 hasConcept C530470458 @default.
- W2110147986 hasConcept C71924100 @default.
- W2110147986 hasConceptScore W2110147986C121608353 @default.
- W2110147986 hasConceptScore W2110147986C126322002 @default.
- W2110147986 hasConceptScore W2110147986C142724271 @default.
- W2110147986 hasConceptScore W2110147986C181152851 @default.
- W2110147986 hasConceptScore W2110147986C203014093 @default.
- W2110147986 hasConceptScore W2110147986C2776217527 @default.
- W2110147986 hasConceptScore W2110147986C2777649267 @default.
- W2110147986 hasConceptScore W2110147986C2777761686 @default.
- W2110147986 hasConceptScore W2110147986C2779720271 @default.
- W2110147986 hasConceptScore W2110147986C2779790080 @default.
- W2110147986 hasConceptScore W2110147986C2780614586 @default.
- W2110147986 hasConceptScore W2110147986C2780849966 @default.
- W2110147986 hasConceptScore W2110147986C2780931953 @default.
- W2110147986 hasConceptScore W2110147986C3018346242 @default.
- W2110147986 hasConceptScore W2110147986C530470458 @default.
- W2110147986 hasConceptScore W2110147986C71924100 @default.
- W2110147986 hasIssue "3" @default.
- W2110147986 hasLocation W21101479861 @default.
- W2110147986 hasLocation W21101479862 @default.
- W2110147986 hasOpenAccess W2110147986 @default.
- W2110147986 hasPrimaryLocation W21101479861 @default.
- W2110147986 hasRelatedWork W1555650801 @default.
- W2110147986 hasRelatedWork W2034240615 @default.
- W2110147986 hasRelatedWork W2056698445 @default.
- W2110147986 hasRelatedWork W2077898287 @default.
- W2110147986 hasRelatedWork W2081826732 @default.
- W2110147986 hasRelatedWork W2089690087 @default.
- W2110147986 hasRelatedWork W2107486514 @default.
- W2110147986 hasRelatedWork W2110147986 @default.
- W2110147986 hasRelatedWork W2163100062 @default.
- W2110147986 hasRelatedWork W579116757 @default.
- W2110147986 hasVolume "257" @default.
- W2110147986 isParatext "false" @default.
- W2110147986 isRetracted "false" @default.
- W2110147986 magId "2110147986" @default.
- W2110147986 workType "article" @default.