Matches in SemOpenAlex for { <https://semopenalex.org/work/W2110198213> ?p ?o ?g. }
- W2110198213 abstract "Ewing's sarcomas are aggressive musculoskeletal tumors occurring most frequently in the long and flat bones as a solitary lesion mostly during the teen-age years of life. With current treatments, significant number of patients relapse and survival is poor for those with metastatic disease. As part of novel target discovery in Ewing's sarcoma, we applied RNAi mediated phenotypic profiling to identify kinase targets involved in growth and survival of Ewing's sarcoma cells.Four Ewing's sarcoma cell lines TC-32, TC-71, SK-ES-1 and RD-ES were tested in high throughput-RNAi screens using a siRNA library targeting 572 kinases. Knockdown of 25 siRNAs reduced the growth of all four Ewing's sarcoma cell lines in replicate screens. Of these, 16 siRNA were specific and reduced proliferation of Ewing's sarcoma cells as compared to normal fibroblasts. Secondary validation and preliminary mechanistic studies highlighted the kinases STK10 and TNK2 as having important roles in growth and survival of Ewing's sarcoma cells. Furthermore, knockdown of STK10 and TNK2 by siRNA showed increased apoptosis.In summary, RNAi-based phenotypic profiling proved to be a powerful gene target discovery strategy, leading to successful identification and validation of STK10 and TNK2 as two novel potential therapeutic targets for Ewing's sarcoma." @default.
- W2110198213 created "2016-06-24" @default.
- W2110198213 creator A5005722897 @default.
- W2110198213 creator A5012734710 @default.
- W2110198213 creator A5018041025 @default.
- W2110198213 creator A5028402481 @default.
- W2110198213 creator A5046903606 @default.
- W2110198213 creator A5054693021 @default.
- W2110198213 creator A5079727798 @default.
- W2110198213 creator A5087252132 @default.
- W2110198213 creator A5044042264 @default.
- W2110198213 date "2010-08-18" @default.
- W2110198213 modified "2023-10-14" @default.
- W2110198213 title "RNAi phenotype profiling of kinases identifies potential therapeutic targets in Ewing's sarcoma" @default.
- W2110198213 cites W1641731911 @default.
- W2110198213 cites W1843078452 @default.
- W2110198213 cites W1967597978 @default.
- W2110198213 cites W1972809568 @default.
- W2110198213 cites W1974899413 @default.
- W2110198213 cites W1980090847 @default.
- W2110198213 cites W1981658281 @default.
- W2110198213 cites W1981717620 @default.
- W2110198213 cites W1987113772 @default.
- W2110198213 cites W2000149489 @default.
- W2110198213 cites W2000966286 @default.
- W2110198213 cites W2020564508 @default.
- W2110198213 cites W2034729360 @default.
- W2110198213 cites W2037139254 @default.
- W2110198213 cites W2040371894 @default.
- W2110198213 cites W2042452896 @default.
- W2110198213 cites W2043085463 @default.
- W2110198213 cites W2051000382 @default.
- W2110198213 cites W2051151149 @default.
- W2110198213 cites W2056561005 @default.
- W2110198213 cites W2060722365 @default.
- W2110198213 cites W2062709744 @default.
- W2110198213 cites W2071451207 @default.
- W2110198213 cites W2072315137 @default.
- W2110198213 cites W2076011370 @default.
- W2110198213 cites W2087046564 @default.
- W2110198213 cites W2088733491 @default.
- W2110198213 cites W2090915824 @default.
- W2110198213 cites W2092828528 @default.
- W2110198213 cites W2093345318 @default.
- W2110198213 cites W2095126639 @default.
- W2110198213 cites W2100263195 @default.
- W2110198213 cites W2101127767 @default.
- W2110198213 cites W2107593804 @default.
- W2110198213 cites W2118471037 @default.
- W2110198213 cites W2125026963 @default.
- W2110198213 cites W2125433806 @default.
- W2110198213 cites W2128683587 @default.
- W2110198213 cites W2138128689 @default.
- W2110198213 cites W2139326294 @default.
- W2110198213 cites W2143742430 @default.
- W2110198213 cites W2150091347 @default.
- W2110198213 cites W2152324247 @default.
- W2110198213 cites W2155824282 @default.
- W2110198213 cites W2164937682 @default.
- W2110198213 cites W2167872282 @default.
- W2110198213 cites W2168955110 @default.
- W2110198213 cites W2266000538 @default.
- W2110198213 cites W2319415470 @default.
- W2110198213 cites W2325387537 @default.
- W2110198213 cites W4253970938 @default.
- W2110198213 doi "https://doi.org/10.1186/1476-4598-9-218" @default.
- W2110198213 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2933621" @default.
- W2110198213 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20718987" @default.
- W2110198213 hasPublicationYear "2010" @default.
- W2110198213 type Work @default.
- W2110198213 sameAs 2110198213 @default.
- W2110198213 citedByCount "27" @default.
- W2110198213 countsByYear W21101982132012 @default.
- W2110198213 countsByYear W21101982132013 @default.
- W2110198213 countsByYear W21101982132015 @default.
- W2110198213 countsByYear W21101982132016 @default.
- W2110198213 countsByYear W21101982132017 @default.
- W2110198213 countsByYear W21101982132018 @default.
- W2110198213 countsByYear W21101982132019 @default.
- W2110198213 countsByYear W21101982132020 @default.
- W2110198213 countsByYear W21101982132021 @default.
- W2110198213 countsByYear W21101982132022 @default.
- W2110198213 crossrefType "journal-article" @default.
- W2110198213 hasAuthorship W2110198213A5005722897 @default.
- W2110198213 hasAuthorship W2110198213A5012734710 @default.
- W2110198213 hasAuthorship W2110198213A5018041025 @default.
- W2110198213 hasAuthorship W2110198213A5028402481 @default.
- W2110198213 hasAuthorship W2110198213A5044042264 @default.
- W2110198213 hasAuthorship W2110198213A5046903606 @default.
- W2110198213 hasAuthorship W2110198213A5054693021 @default.
- W2110198213 hasAuthorship W2110198213A5079727798 @default.
- W2110198213 hasAuthorship W2110198213A5087252132 @default.
- W2110198213 hasBestOaLocation W21101982131 @default.
- W2110198213 hasConcept C104317684 @default.
- W2110198213 hasConcept C127716648 @default.
- W2110198213 hasConcept C142724271 @default.
- W2110198213 hasConcept C166703698 @default.
- W2110198213 hasConcept C173396325 @default.
- W2110198213 hasConcept C184235292 @default.
- W2110198213 hasConcept C22615655 @default.