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- W2110217000 abstract "We describe a nonscanning, fiber bundle endomicroscope that performs optically sectioned fluorescence imaging with fast frame rates and real-time processing. Our sectioning technique is based on HiLo imaging, wherein two widefield images are acquired under uniform and structured illumination and numerically processed to reject out-of-focus background. This work is an improvement upon an earlier demonstration of widefield optical sectioning through a flexible fiber bundle. The improved device features lateral and axial resolutions of 2.6 and 17 μm, respectively, a net frame rate of 9.5 Hz obtained by real-time image processing with a graphics processing unit (GPU) and significantly reduced motion artifacts obtained by the use of a double-shutter camera. We demonstrate the performance of our system with optically sectioned images and videos of a fluorescently labeled chorioallantoic membrane (CAM) in the developing G. gallus embryo. HiLo endomicroscopy is a candidate technique for low-cost, high-speed clinical optical biopsies." @default.
- W2110217000 created "2016-06-24" @default.
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- W2110217000 date "2012-03-13" @default.
- W2110217000 modified "2023-09-24" @default.
- W2110217000 title "Fast optically sectioned fluorescence HiLo endomicroscopy" @default.
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- W2110217000 doi "https://doi.org/10.1117/1.jbo.17.2.021105" @default.
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