Matches in SemOpenAlex for { <https://semopenalex.org/work/W2110244347> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2110244347 endingPage "306" @default.
- W2110244347 startingPage "299" @default.
- W2110244347 abstract "ABSTRACT Published assays that use TaqMan PCR are consistently sensitive, rapid, and readily transferable. Here we describe a TaqMan PCR-based method for the detection of rabies virus (RV) RNA in tissue samples. We show that the method has an acceptable linear range, is both sensitive and specific, and, importantly, correlates with the concentration of infectious virus. In addition, the levels of RV-specific amplification are adjustable according to the levels of an endogenous control (β-actin mRNA), allowing the calculation of comparable quantities. We tested the capacity of this assay to cope with target sequence variations. The number of sequence mismatches between gene-specific oligonucleotides and the target sequence significantly affects amplification ( P < 0.001), and point mutations at the center of the probe can result in false-negative results through the prevention of probe binding and subsequent fluorescence. This study demonstrates that the genetic heterogeneity of RVs may prove a serious obstacle in the development of a diagnostic assay based on TaqMan PCR; however, the quantification of RV levels may prove to be a valuable application of this assay." @default.
- W2110244347 created "2016-06-24" @default.
- W2110244347 creator A5012181714 @default.
- W2110244347 creator A5059693322 @default.
- W2110244347 creator A5060752160 @default.
- W2110244347 creator A5070933734 @default.
- W2110244347 date "2004-01-01" @default.
- W2110244347 modified "2023-09-26" @default.
- W2110244347 title "Evaluation of a TaqMan PCR Assay To Detect Rabies Virus RNA: Influence of Sequence Variation and Application to Quantification of Viral Loads" @default.
- W2110244347 cites W1985481517 @default.
- W2110244347 cites W1989069389 @default.
- W2110244347 cites W1994371904 @default.
- W2110244347 cites W2000442958 @default.
- W2110244347 cites W2003050123 @default.
- W2110244347 cites W2007280898 @default.
- W2110244347 cites W2070141130 @default.
- W2110244347 cites W2102780520 @default.
- W2110244347 cites W2123050502 @default.
- W2110244347 cites W2130637995 @default.
- W2110244347 cites W2136464868 @default.
- W2110244347 cites W2143276927 @default.
- W2110244347 cites W2167835146 @default.
- W2110244347 cites W2169706295 @default.
- W2110244347 cites W2315231115 @default.
- W2110244347 cites W3020963880 @default.
- W2110244347 cites W3021952109 @default.
- W2110244347 doi "https://doi.org/10.1128/jcm.42.1.299-306.2004" @default.
- W2110244347 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/321704" @default.
- W2110244347 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14715769" @default.
- W2110244347 hasPublicationYear "2004" @default.
- W2110244347 type Work @default.
- W2110244347 sameAs 2110244347 @default.
- W2110244347 citedByCount "67" @default.
- W2110244347 countsByYear W21102443472012 @default.
- W2110244347 countsByYear W21102443472013 @default.
- W2110244347 countsByYear W21102443472014 @default.
- W2110244347 countsByYear W21102443472015 @default.
- W2110244347 countsByYear W21102443472016 @default.
- W2110244347 countsByYear W21102443472017 @default.
- W2110244347 countsByYear W21102443472018 @default.
- W2110244347 countsByYear W21102443472020 @default.
- W2110244347 countsByYear W21102443472021 @default.
- W2110244347 countsByYear W21102443472022 @default.
- W2110244347 crossrefType "journal-article" @default.
- W2110244347 hasAuthorship W2110244347A5012181714 @default.
- W2110244347 hasAuthorship W2110244347A5059693322 @default.
- W2110244347 hasAuthorship W2110244347A5060752160 @default.
- W2110244347 hasAuthorship W2110244347A5070933734 @default.
- W2110244347 hasBestOaLocation W21102443471 @default.
- W2110244347 hasConcept C104317684 @default.
- W2110244347 hasConcept C119203544 @default.
- W2110244347 hasConcept C129312508 @default.
- W2110244347 hasConcept C143660080 @default.
- W2110244347 hasConcept C153911025 @default.
- W2110244347 hasConcept C159047783 @default.
- W2110244347 hasConcept C2522874641 @default.
- W2110244347 hasConcept C2776849203 @default.
- W2110244347 hasConcept C2777961786 @default.
- W2110244347 hasConcept C48023723 @default.
- W2110244347 hasConcept C49105822 @default.
- W2110244347 hasConcept C54355233 @default.
- W2110244347 hasConcept C86803240 @default.
- W2110244347 hasConceptScore W2110244347C104317684 @default.
- W2110244347 hasConceptScore W2110244347C119203544 @default.
- W2110244347 hasConceptScore W2110244347C129312508 @default.
- W2110244347 hasConceptScore W2110244347C143660080 @default.
- W2110244347 hasConceptScore W2110244347C153911025 @default.
- W2110244347 hasConceptScore W2110244347C159047783 @default.
- W2110244347 hasConceptScore W2110244347C2522874641 @default.
- W2110244347 hasConceptScore W2110244347C2776849203 @default.
- W2110244347 hasConceptScore W2110244347C2777961786 @default.
- W2110244347 hasConceptScore W2110244347C48023723 @default.
- W2110244347 hasConceptScore W2110244347C49105822 @default.
- W2110244347 hasConceptScore W2110244347C54355233 @default.
- W2110244347 hasConceptScore W2110244347C86803240 @default.
- W2110244347 hasIssue "1" @default.
- W2110244347 hasLocation W21102443471 @default.
- W2110244347 hasLocation W21102443472 @default.
- W2110244347 hasLocation W21102443473 @default.
- W2110244347 hasOpenAccess W2110244347 @default.
- W2110244347 hasPrimaryLocation W21102443471 @default.
- W2110244347 hasRelatedWork W1977371468 @default.
- W2110244347 hasRelatedWork W1995761937 @default.
- W2110244347 hasRelatedWork W2003276919 @default.
- W2110244347 hasRelatedWork W2032868487 @default.
- W2110244347 hasRelatedWork W2040046438 @default.
- W2110244347 hasRelatedWork W2046021971 @default.
- W2110244347 hasRelatedWork W2068146298 @default.
- W2110244347 hasRelatedWork W2110244347 @default.
- W2110244347 hasRelatedWork W2358965301 @default.
- W2110244347 hasRelatedWork W2394234133 @default.
- W2110244347 hasVolume "42" @default.
- W2110244347 isParatext "false" @default.
- W2110244347 isRetracted "false" @default.
- W2110244347 magId "2110244347" @default.
- W2110244347 workType "article" @default.