FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2110287462> ?p ?o ?g. }

• W2110287462 endingPage "2213" @default.
• W2110287462 startingPage "2203" @default.
• W2110287462 abstract "Abstract The role of myoglobin (Mb) is not yet completely understood and recent evidence indicates it is involved in a variety of pseudo‐enzymatic functions. Mb is also extensively used as a tool to redesign novel active‐site features and introduce new activities into a protein. This review summarizes our approach to enhance the peroxidase‐like activity of Mb toward phenolic compounds by combining two different strategies of protein modification, i.e. active‐site engineering and cofactor replacement. The main objective of active‐site engineering is to increase the rate of hydrogen peroxide activation upon reaction at the iron( III ) center, while cofactor replacement facilitates substrate interaction at the protein active site, thereby increasing the efficiency of the catalytic process and stereoselective recognition of the substrate. The thermodynamic stability of the modified Mb derivatives has been evaluated through guanidinium chloride induced and thermal unfolding experiments. As expected, both protein mutation and cofactor replacement decrease the stability of the protein, the latter effect being somewhat more pronounced for the Mb derivatives studied here. For evaluating the catalytic efficiency of the protein in non‐natural reactions, the chemical stability of the protein during catalysis is also important and therefore a new protocol was developed to evaluate the competitive degradation undergone by the protein upon substrate oxidation. It was found that autodegradation can occur through two different pathways involving oxidation of protein residues by the Mb active species or by a substrate‐derived phenoxy radical. The peroxidase‐like nitrating activity and the sulfoxidation reactions promoted by Mb, both involving the nitrite ion as a cosubstrate, have also been briefly described. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2004)" @default.
• W2110287462 created "2016-06-24" @default.
• W2110287462 creator A5026694008 @default.
• W2110287462 creator A5030387781 @default.
• W2110287462 creator A5033857804 @default.
• W2110287462 creator A5064465904 @default.
• W2110287462 date "2004-05-25" @default.
• W2110287462 modified "2023-09-24" @default.
• W2110287462 title "Engineering and Prosthetic‐Group Modification of Myoglobin: Peroxidase Activity, Chemical Stability and Unfolding Properties" @default.
• W2110287462 cites W1496877100 @default.
• W2110287462 cites W1531583716 @default.
• W2110287462 cites W1533569014 @default.
• W2110287462 cites W1578742674 @default.
• W2110287462 cites W1600153356 @default.
• W2110287462 cites W1658740647 @default.
• W2110287462 cites W1725300350 @default.
• W2110287462 cites W1764308599 @default.
• W2110287462 cites W1828125754 @default.
• W2110287462 cites W1963565399 @default.
• W2110287462 cites W1965240815 @default.
• W2110287462 cites W1968872901 @default.
• W2110287462 cites W1972972214 @default.
• W2110287462 cites W1973537942 @default.
• W2110287462 cites W1974358158 @default.
• W2110287462 cites W1977587847 @default.
• W2110287462 cites W1982148500 @default.
• W2110287462 cites W1989282569 @default.
• W2110287462 cites W1990710075 @default.
• W2110287462 cites W1994208230 @default.
• W2110287462 cites W2005483875 @default.
• W2110287462 cites W2005722437 @default.
• W2110287462 cites W2006999117 @default.
• W2110287462 cites W2011047569 @default.
• W2110287462 cites W2013748091 @default.
• W2110287462 cites W2017785367 @default.
• W2110287462 cites W2019534267 @default.
• W2110287462 cites W2021090633 @default.
• W2110287462 cites W2022437845 @default.
• W2110287462 cites W2022478922 @default.
• W2110287462 cites W2023026465 @default.
• W2110287462 cites W2026564861 @default.
• W2110287462 cites W2026662914 @default.
• W2110287462 cites W2027125694 @default.
• W2110287462 cites W2028889672 @default.
• W2110287462 cites W2031070939 @default.
• W2110287462 cites W2032092394 @default.
• W2110287462 cites W2033379487 @default.
• W2110287462 cites W2035532840 @default.
• W2110287462 cites W2038263678 @default.
• W2110287462 cites W2039803809 @default.
• W2110287462 cites W2041062042 @default.
• W2110287462 cites W2041622905 @default.
• W2110287462 cites W2041879090 @default.
• W2110287462 cites W2043579465 @default.
• W2110287462 cites W2043606464 @default.
• W2110287462 cites W2044245086 @default.
• W2110287462 cites W2049661339 @default.
• W2110287462 cites W2056254096 @default.
• W2110287462 cites W2057547050 @default.
• W2110287462 cites W2065035451 @default.
• W2110287462 cites W2066538437 @default.
• W2110287462 cites W2068863008 @default.
• W2110287462 cites W2069680427 @default.
• W2110287462 cites W2071560742 @default.
• W2110287462 cites W2071704022 @default.
• W2110287462 cites W2082125955 @default.
• W2110287462 cites W2085639037 @default.
• W2110287462 cites W2086775365 @default.
• W2110287462 cites W2089369342 @default.
• W2110287462 cites W2089390718 @default.
• W2110287462 cites W2089470865 @default.
• W2110287462 cites W2090302703 @default.
• W2110287462 cites W2096150716 @default.
• W2110287462 cites W2110287462 @default.
• W2110287462 cites W2116835524 @default.
• W2110287462 cites W2128022125 @default.
• W2110287462 cites W2130479394 @default.
• W2110287462 cites W2131643591 @default.
• W2110287462 cites W2137859704 @default.
• W2110287462 cites W2152600458 @default.
• W2110287462 cites W2161183872 @default.
• W2110287462 cites W2162833635 @default.
• W2110287462 cites W2165898505 @default.
• W2110287462 cites W2179092050 @default.
• W2110287462 cites W2344481344 @default.
• W2110287462 cites W2950382359 @default.
• W2110287462 doi "https://doi.org/10.1002/ejic.200400126" @default.
• W2110287462 hasPublicationYear "2004" @default.
• W2110287462 type Work @default.
• W2110287462 sameAs 2110287462 @default.
• W2110287462 citedByCount "18" @default.
• W2110287462 countsByYear W21102874622013 @default.
• W2110287462 countsByYear W21102874622015 @default.
• W2110287462 countsByYear W21102874622022 @default.
• W2110287462 crossrefType "journal-article" @default.
• W2110287462 hasAuthorship W2110287462A5026694008 @default.
• W2110287462 hasAuthorship W2110287462A5030387781 @default.
• W2110287462 hasAuthorship W2110287462A5033857804 @default.

• next