FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2110319746> ?p ?o ?g. }

• W2110319746 endingPage "1693" @default.
• W2110319746 startingPage "1682" @default.
• W2110319746 abstract "Selective deamidation of proteins and peptides is a reaction of great interest, both because it has a physiological role and because it can cause alteration in the biological activity, local folding, and overall stability of the protein. In order to evaluate the thermodynamic effects of this reaction in proteins, we investigated the temperature-induced denaturation of ribonuclease A derivatives in which asparagine 67 was selectively replaced by an aspartyl residue or an isoaspartyl residue, as a consequence of an in vitro deamidation reaction. Differential scanning calorimetry measurements were performed in the pH range 3.0-6.0, where the unfolding process is reversible, according to the reheating criterion used. It resulted that the monodeamidated forms have a different thermal stability with respect to the parent enzyme. In particular, the replacement of asparagine 67 with an isoaspartyl residue leads to a decrease of 6.3 °C of denaturation temperature and 65 kJ mol −1 of denaturation enthalpy at pH 5.0. These results are discussed and correlated to the X-ray three-dimensional structure of this derivative. The analysis leads to the conclusion that the difference in thermal stability between RNase A and (N67isoD)RNase A is due to enthalpic effects arising from the loss of two important hydrogen bonds in the loop containing residue 67, partially counterbalanced by entropic effects. Finally, the influence of cytidine-2′-monophosphate on the stability of the three ribonucleases at pH 5.0 is studied and explained in terms of its binding on the active site of ribonucleases. The analysis makes it possible to estimate the apparent binding constant and binding enthalpy for the three proteins." @default.
• W2110319746 created "2016-06-24" @default.
• W2110319746 creator A5056333763 @default.
• W2110319746 creator A5066386855 @default.
• W2110319746 creator A5070512330 @default.
• W2110319746 creator A5073798207 @default.
• W2110319746 date "1997-08-01" @default.
• W2110319746 modified "2023-09-25" @default.
• W2110319746 title "Thermodynamic analysis of the effect of selective monodeamidation at asparagine 67 in ribonuclease A" @default.
• W2110319746 cites W1040905271 @default.
• W2110319746 cites W1496368635 @default.
• W2110319746 cites W1498140959 @default.
• W2110319746 cites W1529873738 @default.
• W2110319746 cites W1533569014 @default.
• W2110319746 cites W1536128836 @default.
• W2110319746 cites W1585503267 @default.
• W2110319746 cites W1593567129 @default.
• W2110319746 cites W1621212198 @default.
• W2110319746 cites W1820160793 @default.
• W2110319746 cites W1964757220 @default.
• W2110319746 cites W1967795745 @default.
• W2110319746 cites W1970857264 @default.
• W2110319746 cites W1971138313 @default.
• W2110319746 cites W1977308661 @default.
• W2110319746 cites W1985375832 @default.
• W2110319746 cites W1985795838 @default.
• W2110319746 cites W1988670311 @default.
• W2110319746 cites W1994785538 @default.
• W2110319746 cites W1996416376 @default.
• W2110319746 cites W1997778077 @default.
• W2110319746 cites W2001209696 @default.
• W2110319746 cites W2004421107 @default.
• W2110319746 cites W2004773817 @default.
• W2110319746 cites W2005956300 @default.
• W2110319746 cites W2006027828 @default.
• W2110319746 cites W2015510077 @default.
• W2110319746 cites W2015883683 @default.
• W2110319746 cites W2016497865 @default.
• W2110319746 cites W2016907315 @default.
• W2110319746 cites W2019374625 @default.
• W2110319746 cites W2020357919 @default.
• W2110319746 cites W2025049563 @default.
• W2110319746 cites W2025773262 @default.
• W2110319746 cites W2025925640 @default.
• W2110319746 cites W2030051164 @default.
• W2110319746 cites W2032899658 @default.
• W2110319746 cites W2035594922 @default.
• W2110319746 cites W2040539292 @default.
• W2110319746 cites W2046033652 @default.
• W2110319746 cites W2053621368 @default.
• W2110319746 cites W2054019055 @default.
• W2110319746 cites W2056882760 @default.
• W2110319746 cites W2060868573 @default.
• W2110319746 cites W2061124331 @default.
• W2110319746 cites W2064168223 @default.
• W2110319746 cites W2066337561 @default.
• W2110319746 cites W2067392322 @default.
• W2110319746 cites W2070541988 @default.
• W2110319746 cites W2070783701 @default.
• W2110319746 cites W2073138345 @default.
• W2110319746 cites W2073662901 @default.
• W2110319746 cites W2081411678 @default.
• W2110319746 cites W2087384049 @default.
• W2110319746 cites W2101971141 @default.
• W2110319746 cites W2104017463 @default.
• W2110319746 cites W2105184766 @default.
• W2110319746 cites W2150725533 @default.
• W2110319746 cites W2158262572 @default.
• W2110319746 cites W2159392498 @default.
• W2110319746 cites W2166924299 @default.
• W2110319746 cites W2410442637 @default.
• W2110319746 cites W2413485770 @default.
• W2110319746 cites W2418945804 @default.
• W2110319746 cites W2419014510 @default.
• W2110319746 cites W28039676 @default.
• W2110319746 doi "https://doi.org/10.1002/pro.5560060808" @default.
• W2110319746 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2143771" @default.
• W2110319746 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9260280" @default.
• W2110319746 hasPublicationYear "1997" @default.
• W2110319746 type Work @default.
• W2110319746 sameAs 2110319746 @default.
• W2110319746 citedByCount "44" @default.
• W2110319746 countsByYear W21103197462012 @default.
• W2110319746 countsByYear W21103197462013 @default.
• W2110319746 countsByYear W21103197462016 @default.
• W2110319746 countsByYear W21103197462017 @default.
• W2110319746 countsByYear W21103197462018 @default.
• W2110319746 countsByYear W21103197462020 @default.
• W2110319746 countsByYear W21103197462022 @default.
• W2110319746 countsByYear W21103197462023 @default.
• W2110319746 crossrefType "journal-article" @default.
• W2110319746 hasAuthorship W2110319746A5056333763 @default.
• W2110319746 hasAuthorship W2110319746A5066386855 @default.
• W2110319746 hasAuthorship W2110319746A5070512330 @default.
• W2110319746 hasAuthorship W2110319746A5073798207 @default.
• W2110319746 hasBestOaLocation W21103197462 @default.
• W2110319746 hasConcept C104317684 @default.
• W2110319746 hasConcept C10879258 @default.

• next