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• W2110332392 abstract "Abstract The use of stable isotope labelled glucose provides insight into glucose metabolism. The 13 C‐isotopic enrichment of glucose is usually measured by gas chromatography/mass spectrometry (GC/MS) or gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS). However, in both techniques the samples must be derivatized prior to analysis, which makes sample preparation more labour‐intensive and increases the uncertainty of the measured isotopic composition. A novel method for the determination of isotopic enrichment of glucose in human plasma using liquid chromatography/isotope ratio mass spectrometry (LC/IRMS) has been developed. Using this technique, for which hardly any sample preparation is needed, we showed that both the enrichment and the concentration could be measured with very high precision using only 20 µL of plasma. In addition, a comparison with GC/MS and GC/IRMS showed that the best performance was achieved with the LC/IRMS method making it the method of choice for the measurement of 13 C‐isotopic enrichment in plasma samples. Copyright © 2009 John Wiley & Sons, Ltd." @default.
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• W2110332392 date "2009-11-09" @default.
• W2110332392 modified "2023-10-15" @default.
• W2110332392 title "Analysis of [U-¹³ C₆ ]glucose in human plasma using liquid chromatography/isotope ratio mass spectrometry compared with two other mass spectrometry techniques" @default.
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• W2110332392 doi "https://doi.org/10.1002/rcm.4293" @default.
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