Matches in SemOpenAlex for { <https://semopenalex.org/work/W2110339917> ?p ?o ?g. }
- W2110339917 endingPage "765" @default.
- W2110339917 startingPage "757" @default.
- W2110339917 abstract "The HasA(SM) hemophore, secreted by Serratia marcescens, binds free or hemoprotein bound heme with high affinity and delivers it to a specific outer membrane receptor, HasR. In HasA(SM), heme is held by two loops and coordinated to iron by two residues, His 32 and Tyr 75. A third residue His 83 was shown recently to play a crucial role in heme ligation. To address the mechanistic issues of the heme capture and release processes, the histidine protonation states were studied in both apo- and holo-forms of HasA(SM) in solution. Holo-HasA(SM) was formed with gallium-protoporphyrin IX (GaPPIX), giving rise to a diamagnetic protein. By use of heteronuclear correlation NMR spectroscopy, the imidazole side-chain (15)N and (1)H resonances of the six HasA(SM) histidines were assigned and their pKa values and predominant tautomeric states according to pH were determined. We show that protonation states of the heme pocket histidines can modulate the nucleophilic character of the two axial ligands and, consequently, control the heme binding. In particular, the essential role of the His 83 is emphasized according to its direct interaction with Tyr 75." @default.
- W2110339917 created "2016-06-24" @default.
- W2110339917 creator A5010607158 @default.
- W2110339917 creator A5019310344 @default.
- W2110339917 creator A5021853344 @default.
- W2110339917 creator A5024026681 @default.
- W2110339917 creator A5045852008 @default.
- W2110339917 creator A5052538879 @default.
- W2110339917 creator A5068728973 @default.
- W2110339917 creator A5070531752 @default.
- W2110339917 creator A5072693404 @default.
- W2110339917 date "2002-04-01" @default.
- W2110339917 modified "2023-10-09" @default.
- W2110339917 title "Histidine pKa shifts and changes of tautomeric states induced by the binding of gallium-protoporphyrin IX in the hemophore HasASM" @default.
- W2110339917 cites W1505374905 @default.
- W2110339917 cites W1539645325 @default.
- W2110339917 cites W1565821928 @default.
- W2110339917 cites W1566515137 @default.
- W2110339917 cites W1582760489 @default.
- W2110339917 cites W1965731933 @default.
- W2110339917 cites W1973760133 @default.
- W2110339917 cites W1982155086 @default.
- W2110339917 cites W1996939437 @default.
- W2110339917 cites W2013031563 @default.
- W2110339917 cites W2015811409 @default.
- W2110339917 cites W2017765211 @default.
- W2110339917 cites W2028133510 @default.
- W2110339917 cites W2032145697 @default.
- W2110339917 cites W2043757220 @default.
- W2110339917 cites W2044611444 @default.
- W2110339917 cites W2069734146 @default.
- W2110339917 cites W2070446927 @default.
- W2110339917 cites W2080290351 @default.
- W2110339917 cites W2094232976 @default.
- W2110339917 cites W2097844708 @default.
- W2110339917 cites W2113305715 @default.
- W2110339917 cites W2115525854 @default.
- W2110339917 cites W2161425804 @default.
- W2110339917 cites W2165924676 @default.
- W2110339917 cites W22306840 @default.
- W2110339917 cites W2421887775 @default.
- W2110339917 cites W2461459768 @default.
- W2110339917 cites W2785084758 @default.
- W2110339917 cites W2921073505 @default.
- W2110339917 cites W4245786059 @default.
- W2110339917 cites W51268287 @default.
- W2110339917 cites W998400604 @default.
- W2110339917 doi "https://doi.org/10.1110/ps.3630102" @default.
- W2110339917 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2373534" @default.
- W2110339917 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11910020" @default.
- W2110339917 hasPublicationYear "2002" @default.
- W2110339917 type Work @default.
- W2110339917 sameAs 2110339917 @default.
- W2110339917 citedByCount "53" @default.
- W2110339917 countsByYear W21103399172012 @default.
- W2110339917 countsByYear W21103399172013 @default.
- W2110339917 countsByYear W21103399172014 @default.
- W2110339917 countsByYear W21103399172015 @default.
- W2110339917 countsByYear W21103399172016 @default.
- W2110339917 countsByYear W21103399172017 @default.
- W2110339917 countsByYear W21103399172018 @default.
- W2110339917 countsByYear W21103399172019 @default.
- W2110339917 countsByYear W21103399172020 @default.
- W2110339917 countsByYear W21103399172021 @default.
- W2110339917 countsByYear W21103399172022 @default.
- W2110339917 countsByYear W21103399172023 @default.
- W2110339917 crossrefType "journal-article" @default.
- W2110339917 hasAuthorship W2110339917A5010607158 @default.
- W2110339917 hasAuthorship W2110339917A5019310344 @default.
- W2110339917 hasAuthorship W2110339917A5021853344 @default.
- W2110339917 hasAuthorship W2110339917A5024026681 @default.
- W2110339917 hasAuthorship W2110339917A5045852008 @default.
- W2110339917 hasAuthorship W2110339917A5052538879 @default.
- W2110339917 hasAuthorship W2110339917A5068728973 @default.
- W2110339917 hasAuthorship W2110339917A5070531752 @default.
- W2110339917 hasAuthorship W2110339917A5072693404 @default.
- W2110339917 hasBestOaLocation W21103399172 @default.
- W2110339917 hasConcept C111233374 @default.
- W2110339917 hasConcept C145148216 @default.
- W2110339917 hasConcept C178790620 @default.
- W2110339917 hasConcept C181199279 @default.
- W2110339917 hasConcept C185592680 @default.
- W2110339917 hasConcept C20705724 @default.
- W2110339917 hasConcept C2776217839 @default.
- W2110339917 hasConcept C2777723881 @default.
- W2110339917 hasConcept C2778460671 @default.
- W2110339917 hasConcept C2779671804 @default.
- W2110339917 hasConcept C2780874372 @default.
- W2110339917 hasConcept C2781323092 @default.
- W2110339917 hasConcept C30095370 @default.
- W2110339917 hasConcept C515207424 @default.
- W2110339917 hasConcept C55493867 @default.
- W2110339917 hasConcept C66974803 @default.
- W2110339917 hasConcept C71240020 @default.
- W2110339917 hasConcept C8010536 @default.
- W2110339917 hasConceptScore W2110339917C111233374 @default.
- W2110339917 hasConceptScore W2110339917C145148216 @default.
- W2110339917 hasConceptScore W2110339917C178790620 @default.