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• W2110386681 abstract "Abstract Synthetic biodegradable polymers have many potential therapeutic applications. In ophthalmology, biodegradable polymers have been used as viscoelastic agents and surgical implants. Other potential applications include controlled release of drugs and growth factors, gene therapy, and tissue engineering. In the present study, in vitro biocompatibility of three biodegradable polymers, 50:50 PDLGA, 85:15 PDLGA, and Inion GTR™ membrane was evaluated in comparison to tissue culture polystyrene by investigating cell proliferation and potential acute toxicity by the WST‐1 cytotoxicity/cell proliferation test, the ATP test, and the lactate dehydrogenase (LDH) test. Evaluations were conducted with cell line cultures from various ocular tissues, human corneal epithelial cells (HCE), rabbit stromal fibroblasts (SIRC), bovine corneal endothelial cells (BCE), human conjunctival epithelial cells (IOBA‐NHC), and human retinal pigment epithelial cells (ARPE‐19) by direct contact studies by plating the cells on the polymer film specimens in 96‐wells. The proliferation results show that cell lines from various ocular tissues attached and grew on PDLGA 50:50, PDLGA 85:15, and Inion GTR™ membrane. Cytotoxicity experiments with the LDH and ATP tests showed no or extremely slight toxic adverse effects. These polymers have potential to be used as scaffolds in cell transplantation devices or as surgical implants. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2007" @default.
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• W2110386681 date "2007-04-20" @default.
• W2110386681 modified "2023-10-17" @default.
• W2110386681 title "In vitro biocompatibility of degradable biopolymers in cell line cultures from various ocular tissues: Direct contact studies" @default.
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• W2110386681 doi "https://doi.org/10.1002/jbm.a.31319" @default.
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