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• W2110399619 endingPage "1912" @default.
• W2110399619 startingPage "1902" @default.
• W2110399619 abstract "When oligonucleotide probes are microinjected into cells to image the distribution of RNAs, they are rapidly sequestered into the nucleus. As a result, it is difficult to detect mRNAs in the cytoplasm of living cells. We were able to overcome this process by attaching tRNA transcripts to the probes. We show that when fluorescently labeled tRNAs, tRNAs with extensions at their 5′ end, or chimeric molecules in which a molecular beacon possessing a 2′- O -methylribonucleotide backbone is linked to a tRNA, are injected into the nucleus of HeLa cells, they are exported into the cytoplasm. When these constructs are introduced into the cytoplasm, they remain cytoplasmic. These constructs allow the distribution of both the general mRNA population and specific mRNAs to be imaged in living cells. This strategy should also be useful for enhancing the efficacy of antisense oligonucleotides by keeping them in the cytoplasm. Our observations show that the fidelity of the tRNA export system is relaxed for unnatural tRNA variants when they are introduced into the nucleus in large amounts." @default.
• W2110399619 created "2016-06-24" @default.
• W2110399619 creator A5058789705 @default.
• W2110399619 date "2005-03-23" @default.
• W2110399619 modified "2023-10-11" @default.
• W2110399619 title "tRNA-linked molecular beacons for imaging mRNAs in the cytoplasm of living cells" @default.
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• W2110399619 doi "https://doi.org/10.1093/nar/gki302" @default.
• W2110399619 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1074395" @default.
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• W2110399619 hasPublicationYear "2005" @default.
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