Matches in SemOpenAlex for { <https://semopenalex.org/work/W2110441995> ?p ?o ?g. }
- W2110441995 endingPage "67" @default.
- W2110441995 startingPage "67e" @default.
- W2110441995 abstract "A simple and effective modified ethanol precipitation-based protocol is described for the preparation of DNA from ancient human bones. This method is fast and requires neither hazardous chemicals nor special devices. After the powdering and incubating of the bone samples Dextran Blue was added as a carrier for removing the PCR inhibitors with selective ethanol precipitation. This method could eliminate the time-consuming separate decalcification step, dialysis, application of centrifugation-driven microconcentrators and the second consecutive PCR amplification. The efficiency of this procedure was demonstrated on ten 500–1200-year-old human bones from four different Hungarian burial sites. A mitochondrial specific primer pair was used to obtain sequence information from the purified ancient DNA. The PCR amplification, after our DNA extraction protocol, was successful from each of the 10 bone samples investigated. The results demonstrate that extraction of DNA from ancient bone samples with this new approach increases the success rate of PCR amplification." @default.
- W2110441995 created "2016-06-24" @default.
- W2110441995 creator A5005647076 @default.
- W2110441995 creator A5042737097 @default.
- W2110441995 creator A5083480894 @default.
- W2110441995 creator A5085684633 @default.
- W2110441995 date "2000-06-15" @default.
- W2110441995 modified "2023-10-18" @default.
- W2110441995 title "A simple and efficient method for PCR amplifiable DNA extraction from ancient bones" @default.
- W2110441995 cites W1027715670 @default.
- W2110441995 cites W119223626 @default.
- W2110441995 cites W1604394245 @default.
- W2110441995 cites W1605768452 @default.
- W2110441995 cites W1662982009 @default.
- W2110441995 cites W1776425896 @default.
- W2110441995 cites W1834685305 @default.
- W2110441995 cites W1982950335 @default.
- W2110441995 cites W1996781317 @default.
- W2110441995 cites W1998948401 @default.
- W2110441995 cites W2008586684 @default.
- W2110441995 cites W2030811936 @default.
- W2110441995 cites W2046960985 @default.
- W2110441995 cites W2049454421 @default.
- W2110441995 cites W2052350257 @default.
- W2110441995 cites W2090684975 @default.
- W2110441995 cites W2093798885 @default.
- W2110441995 cites W2109291482 @default.
- W2110441995 cites W2116936423 @default.
- W2110441995 cites W2124593896 @default.
- W2110441995 cites W2134259738 @default.
- W2110441995 cites W2155288921 @default.
- W2110441995 cites W2156380818 @default.
- W2110441995 cites W2165582585 @default.
- W2110441995 cites W288330647 @default.
- W2110441995 cites W612373117 @default.
- W2110441995 doi "https://doi.org/10.1093/nar/28.12.e67" @default.
- W2110441995 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/102752" @default.
- W2110441995 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10871390" @default.
- W2110441995 hasPublicationYear "2000" @default.
- W2110441995 type Work @default.
- W2110441995 sameAs 2110441995 @default.
- W2110441995 citedByCount "122" @default.
- W2110441995 countsByYear W21104419952012 @default.
- W2110441995 countsByYear W21104419952013 @default.
- W2110441995 countsByYear W21104419952014 @default.
- W2110441995 countsByYear W21104419952015 @default.
- W2110441995 countsByYear W21104419952016 @default.
- W2110441995 countsByYear W21104419952017 @default.
- W2110441995 countsByYear W21104419952018 @default.
- W2110441995 countsByYear W21104419952019 @default.
- W2110441995 countsByYear W21104419952020 @default.
- W2110441995 countsByYear W21104419952021 @default.
- W2110441995 countsByYear W21104419952022 @default.
- W2110441995 countsByYear W21104419952023 @default.
- W2110441995 crossrefType "journal-article" @default.
- W2110441995 hasAuthorship W2110441995A5005647076 @default.
- W2110441995 hasAuthorship W2110441995A5042737097 @default.
- W2110441995 hasAuthorship W2110441995A5083480894 @default.
- W2110441995 hasAuthorship W2110441995A5085684633 @default.
- W2110441995 hasBestOaLocation W21104419951 @default.
- W2110441995 hasConcept C104317684 @default.
- W2110441995 hasConcept C142724271 @default.
- W2110441995 hasConcept C144024400 @default.
- W2110441995 hasConcept C149923435 @default.
- W2110441995 hasConcept C153911025 @default.
- W2110441995 hasConcept C178790620 @default.
- W2110441995 hasConcept C185592680 @default.
- W2110441995 hasConcept C189338579 @default.
- W2110441995 hasConcept C24586158 @default.
- W2110441995 hasConcept C26905121 @default.
- W2110441995 hasConcept C2777563447 @default.
- W2110441995 hasConcept C2780161600 @default.
- W2110441995 hasConcept C2908647359 @default.
- W2110441995 hasConcept C43617362 @default.
- W2110441995 hasConcept C4725764 @default.
- W2110441995 hasConcept C49105822 @default.
- W2110441995 hasConcept C53941386 @default.
- W2110441995 hasConcept C552990157 @default.
- W2110441995 hasConcept C55493867 @default.
- W2110441995 hasConcept C71924100 @default.
- W2110441995 hasConcept C7623868 @default.
- W2110441995 hasConcept C78063203 @default.
- W2110441995 hasConcept C86803240 @default.
- W2110441995 hasConceptScore W2110441995C104317684 @default.
- W2110441995 hasConceptScore W2110441995C142724271 @default.
- W2110441995 hasConceptScore W2110441995C144024400 @default.
- W2110441995 hasConceptScore W2110441995C149923435 @default.
- W2110441995 hasConceptScore W2110441995C153911025 @default.
- W2110441995 hasConceptScore W2110441995C178790620 @default.
- W2110441995 hasConceptScore W2110441995C185592680 @default.
- W2110441995 hasConceptScore W2110441995C189338579 @default.
- W2110441995 hasConceptScore W2110441995C24586158 @default.
- W2110441995 hasConceptScore W2110441995C26905121 @default.
- W2110441995 hasConceptScore W2110441995C2777563447 @default.
- W2110441995 hasConceptScore W2110441995C2780161600 @default.
- W2110441995 hasConceptScore W2110441995C2908647359 @default.
- W2110441995 hasConceptScore W2110441995C43617362 @default.
- W2110441995 hasConceptScore W2110441995C4725764 @default.