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- W2110481951 endingPage "2827" @default.
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- W2110481951 abstract "The Saccharomyces cerevisiae gene PHO5 is an excellent system with which to study regulated changes in chromatin structure. The PHO5 promoter is packaged into four positioned nucleosomes under repressing conditions; upon induction, the structure of these nucleosomes is altered such that the promoter DNA becomes accessible to nucleases. We report here the development and characterization of an in vitro system in which partially purified PHO5 minichromosomes undergo promoter chromatin remodeling. Several hallmarks of thePHO5 chromatin transition in vivo were reproduced in this system. Chromatin remodeling of PHO5minichromosomes required the transcription factors Pho4 and Pho2, was localized to the promoter region of PHO5, and was independent of the chromatin-remodeling complex Swi-Snf. In vitro chromatin remodeling also required the addition of fractionated nuclear extract and hydrolyzable ATP. This in vitro system should serve as a useful tool for identifying the components required for this reaction and for elucidating the mechanism by which the PHO5promoter chromatin structure is changed." @default.
- W2110481951 created "2016-06-24" @default.
- W2110481951 creator A5027386396 @default.
- W2110481951 creator A5033920261 @default.
- W2110481951 date "1999-04-01" @default.
- W2110481951 modified "2023-09-27" @default.
- W2110481951 title "An In Vitro System Recapitulates Chromatin Remodeling at the <i>PHO5</i> Promoter" @default.
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- W2110481951 doi "https://doi.org/10.1128/mcb.19.4.2817" @default.
- W2110481951 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/84074" @default.
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