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- W2110517246 abstract "Summary Seed oil composed of wax esters with long‐chain monoenoic acyl moieties represents a high‐value commodity for industry. Such plant‐derived sperm oil‐like liquid wax esters are biodegradable and can have excellent properties for lubrication. In addition, wax ester oil may represent a superior substrate for biodiesel production. In this study, we demonstrate that the low‐input oil seed crop Camelina sativa can serve as a biotechnological platform for environmentally benign wax ester production. Two biosynthetic steps catalysed by a fatty alcohol‐forming acyl‐CoA reductase ( FAR ) and a wax ester synthase ( WS ) are sufficient to achieve wax ester accumulation from acyl‐CoA substrates. To produce plant‐derived sperm oil‐like liquid wax esters, the WS from Mus musculus ( Mm WS ) or Simmondsia chinensis ( Sc WS ) were expressed in combination with the FAR from Mus musculus ( Mm FAR 1) or Marinobacter aquaeolei ( Ma FAR ) in seeds of Arabidopsis thaliana and Camelina sativa . The three analysed enzyme combinations Oleo3: mC herry: Mm FAR 1∆c/Oleo3: EYFP : Mm WS , Oleo3: mC herry: Mm FAR 1∆c/ Sc WS and Ma FAR / Sc WS showed differences in the wax ester molecular species profiles and overall biosynthetic performance. By expressing Ma FAR / Sc WS in Arabidopsis or Camelina up to 59% or 21% of the seed oil TAG s were replaced by wax esters, respectively. This combination also yielded wax ester molecular species with highest content of monounsaturated acyl moieties. Expression of the enzyme combinations in the Arabidopsis fae1 fad2 mutant background high in oleic acid resulted in wax ester accumulation enriched in oleyl oleate (18:1/18:1 > 60%), suggesting that similar values may be obtained with a Camelina high oleic acid line." @default.
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- W2110517246 date "2015-04-24" @default.
- W2110517246 modified "2023-10-10" @default.
- W2110517246 title "Synthesis of oleyl oleate wax esters in<i>Arabidopsis thaliana</i>and<i>Camelina sativa</i>seed oil" @default.
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- W2110517246 doi "https://doi.org/10.1111/pbi.12379" @default.
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