FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2110534983> ?p ?o ?g. }

• W2110534983 endingPage "36" @default.
• W2110534983 startingPage "36" @default.
• W2110534983 abstract "During human pregnancy, the production of 17-beta-estradiol (E2) rises steadily to eighty fold at term, and placenta has been found to specifically bind estrogens. We have recently demonstrated the expression of estrogen receptor alpha (ER-alpha) protein in human placenta and its localization in villous cytotrophoblast (CT), vascular pericytes, and amniotic fibroblasts. In vitro, E2 stimulated development of large syncytiotrophoblast (ST) aggregates. In the present study we utilized ER-beta affinity purified polyclonal (N19:sc6820) and ER-alpha monoclonal (clone h-151) antibodies. Western blot analysis revealed a single approximately 52 kDa ER-beta band in chorionic villi (CV) protein extracts. In CV, strong cytoplasmic ER-beta immunoreactivity was confined to ST. Dual color immunohistochemistry revealed asymmetric segregation of ER-alpha in dividing villous CT cells. Prior to separation, the cell nuclei more distant from ST exhibited high ER-alpha, while cell nuclei associated with ST showed diminution of ER-alpha and appearance of ER-beta. In trophoblast cultures, development of ST aggregates was associated with diminution of ER-alpha and appearance of ER-beta immunoreactivity. ER-beta was also detected in endothelial cells, amniotic epithelial cells and fibroblasts, extravillous trophoblast (nuclear and cytoplasmic) and decidual cells (cytoplasmic only). In addition, CFK-E12 (E12) and CWK-F12 (F12) monoclonal antibodies, which recognize approximately 64 kDa ER-beta with hormone binding domain, showed nuclear-specific reactivity with villous ST, extravillous trophoblast, and amniotic epithelium and fibroblasts. Western blot analysis indicated abundant expression of a approximately 64 kDa ER-beta variant in trophoblast cultures, significantly higher when compared to the chorionic villi and freshly isolated trophoblast cell protein extracts. This is the first report on ER-beta expression in human placenta and cultured trophoblast. Our data indicate that during trophoblast differentiation, the ER-alpha is associated with a less, and ER-beta with the more differentiated state. Enhanced expression of approximately 64 kDa ER-beta variant in trophoblast cultures suggests a unique role of ER-beta hormone binding domain in the regulation of trophoblast differentiation. Our data also indicate that asymmetric segregation of ER-alpha may play a role in asymmetric division of estrogen-dependent cells." @default.
• W2110534983 created "2016-06-24" @default.
• W2110534983 creator A5008493509 @default.
• W2110534983 creator A5012039134 @default.
• W2110534983 creator A5015576680 @default.
• W2110534983 creator A5021533182 @default.
• W2110534983 creator A5021728410 @default.
• W2110534983 creator A5022840249 @default.
• W2110534983 creator A5058073900 @default.
• W2110534983 creator A5088673821 @default.
• W2110534983 date "2003-01-01" @default.
• W2110534983 modified "2023-09-27" @default.
• W2110534983 cites W1494693924 @default.
• W2110534983 cites W1505555004 @default.
• W2110534983 cites W1552799192 @default.
• W2110534983 cites W1598493997 @default.
• W2110534983 cites W1772980645 @default.
• W2110534983 cites W1963647531 @default.
• W2110534983 cites W1969961774 @default.
• W2110534983 cites W1970685059 @default.
• W2110534983 cites W1970685458 @default.
• W2110534983 cites W1976710431 @default.
• W2110534983 cites W1980743267 @default.
• W2110534983 cites W1986371906 @default.
• W2110534983 cites W1988001774 @default.
• W2110534983 cites W1989728273 @default.
• W2110534983 cites W1989963009 @default.
• W2110534983 cites W1992389626 @default.
• W2110534983 cites W1995235768 @default.
• W2110534983 cites W1997173362 @default.
• W2110534983 cites W2002372679 @default.
• W2110534983 cites W2004113171 @default.
• W2110534983 cites W2012688990 @default.
• W2110534983 cites W2013950325 @default.
• W2110534983 cites W2022645933 @default.
• W2110534983 cites W2022888607 @default.
• W2110534983 cites W2026271136 @default.
• W2110534983 cites W2038599327 @default.
• W2110534983 cites W2041417274 @default.
• W2110534983 cites W2042256553 @default.
• W2110534983 cites W2053216321 @default.
• W2110534983 cites W2057289143 @default.
• W2110534983 cites W2063377902 @default.
• W2110534983 cites W2064482843 @default.
• W2110534983 cites W2065535202 @default.
• W2110534983 cites W2065643273 @default.
• W2110534983 cites W2065808443 @default.
• W2110534983 cites W2070765757 @default.
• W2110534983 cites W2074169649 @default.
• W2110534983 cites W2078426345 @default.
• W2110534983 cites W2099235053 @default.
• W2110534983 cites W2103963876 @default.
• W2110534983 cites W2114524395 @default.
• W2110534983 cites W2119916207 @default.
• W2110534983 cites W2120209836 @default.
• W2110534983 cites W2126773646 @default.
• W2110534983 cites W2129854423 @default.
• W2110534983 cites W2142418810 @default.
• W2110534983 cites W2145643467 @default.
• W2110534983 cites W2164121800 @default.
• W2110534983 cites W2165347331 @default.
• W2110534983 cites W2167503858 @default.
• W2110534983 cites W2410335316 @default.
• W2110534983 cites W2419192788 @default.
• W2110534983 cites W4298214199 @default.
• W2110534983 doi "https://doi.org/10.1186/1477-7827-1-36" @default.
• W2110534983 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/155643" @default.
• W2110534983 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12740031" @default.
• W2110534983 hasPublicationYear "2003" @default.
• W2110534983 type Work @default.
• W2110534983 sameAs 2110534983 @default.
• W2110534983 citedByCount "82" @default.
• W2110534983 countsByYear W21105349832012 @default.
• W2110534983 countsByYear W21105349832013 @default.
• W2110534983 countsByYear W21105349832014 @default.
• W2110534983 countsByYear W21105349832015 @default.
• W2110534983 countsByYear W21105349832016 @default.
• W2110534983 countsByYear W21105349832017 @default.
• W2110534983 countsByYear W21105349832018 @default.
• W2110534983 countsByYear W21105349832019 @default.
• W2110534983 countsByYear W21105349832020 @default.
• W2110534983 countsByYear W21105349832021 @default.
• W2110534983 countsByYear W21105349832022 @default.
• W2110534983 countsByYear W21105349832023 @default.
• W2110534983 crossrefType "journal-article" @default.
• W2110534983 hasAuthorship W2110534983A5008493509 @default.
• W2110534983 hasAuthorship W2110534983A5012039134 @default.
• W2110534983 hasAuthorship W2110534983A5015576680 @default.
• W2110534983 hasAuthorship W2110534983A5021533182 @default.
• W2110534983 hasAuthorship W2110534983A5021728410 @default.
• W2110534983 hasAuthorship W2110534983A5022840249 @default.
• W2110534983 hasAuthorship W2110534983A5058073900 @default.
• W2110534983 hasAuthorship W2110534983A5088673821 @default.
• W2110534983 hasBestOaLocation W21105349831 @default.
• W2110534983 hasConcept C104317684 @default.
• W2110534983 hasConcept C126322002 @default.
• W2110534983 hasConcept C134018914 @default.
• W2110534983 hasConcept C153911025 @default.

• next