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- W2110549983 abstract "A one-step phosphoryl derivatization method has been used in a peptide sequencing procedure for electrospray ionization tandem mass spectrometry (ESI-MS/MS). The sodiated derivatized peptides exhibit very simple dissociation patterns, in which two kinds of fragment ions, [b(n) + OH + Na]+ and [a(n) + Na]+, are formed. Since the amino acid residues are lost sequentially from the C-terminus, peptide sequences can be identified easily. The fragmentation efficiency of peptides increased as a result of the phosphorylation, and also provided peaks of useful intensity at lower m/z. A peptide with lysine at the C-terminus was derivatized and analyzed by ESI-MS/MS. Similar mass spectra, from which the sequence could be read out, were obtained. This is a novel derivatization method yielding neutral derivatives that should be suitable for peptide sequencing by LC/ESI-MS/MS." @default.
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- W2110549983 date "2002-02-04" @default.
- W2110549983 modified "2023-10-09" @default.
- W2110549983 title "Novel phosphoryl derivatization method for peptide sequencing by electrospray ionization mass spectrometry" @default.
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- W2110549983 doi "https://doi.org/10.1002/rcm.604" @default.
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