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• W2110552918 endingPage "116" @default.
• W2110552918 startingPage "110" @default.
• W2110552918 abstract "Seven-day-old CD-1 mice born to seronegative dams were orally inoculated with a mixture of wild-type simian rotavirus SA11 and wild-type rhesus rotavirus RRV. At various times postinfection, progeny clones were randomly isolated from intestinal homogenates by limiting dilution. Analysis of genome RNAs by polyacrylamide gel electrophoresis was used to identify and genotype reassortant progeny. Reassortment of genome segments was observed in 252 of 662 (38%) clones analyzed from in vivo mixed infections. Kinetic studies indicated that reassortment was an early event in the in vivo infectious cycle; more than 25% of the progeny clones were reassortant by 12 h postinfection. The frequency of reassortant progeny increased to 80 to 100% by 72 to 96 h postinfection. A few reassortants with specific constellations of SA11 and RRV genome segments were repeatedly isolated from different litters or different animals within single litters, suggesting that these genotypes were independently and specifically selected in vivo. Analysis of segregation of individual genome segments among the 252 reassortant progeny revealed that, although most segments segregated randomly, segments 3 and 5 nonrandomly segregated from the SA11 parent. The possible selective pressures active during in vivo reassortment of rotavirus genome segments are discussed." @default.
• W2110552918 created "2016-06-24" @default.
• W2110552918 creator A5025152973 @default.
• W2110552918 creator A5061720887 @default.
• W2110552918 date "1986-01-01" @default.
• W2110552918 modified "2023-09-24" @default.
• W2110552918 title "Analysis of reassortment of genome segments in mice mixedly infected with rotaviruses SA11 and RRV" @default.
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• W2110552918 doi "https://doi.org/10.1128/jvi.57.1.110-116.1986" @default.
• W2110552918 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/252704" @default.
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