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• W2110572406 abstract "Abstract Cyc1 mutants of the yeast Saccharomyces cerevisiae were directly transformed with both sense and antisense oligonucleotides to examine the involvement of the two genomic DNA strands in transformation. Sense oligonucleotides yielded approximately 20-fold more transformants than antisense oligonucleotides. This differential effect was observed with oligonucleotides designed to make alterations at six different sites along the gene and was independent of the oligonucleotide sequence and length, number of mismatches and the host strain. Competition studies showed that antisense oligonucleotides did not inhibit transformation. Although the mechanism for this strand specificity is unknown, this difference was maintained even when CYC1 transcription was diminished to approximately 2% of the normal level." @default.
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• W2110572406 date "1992-08-01" @default.
• W2110572406 modified "2023-10-14" @default.
• W2110572406 title "Strand-specificity in the transformation of yeast with synthetic oligonucleotides." @default.
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• W2110572406 doi "https://doi.org/10.1093/genetics/131.4.811" @default.
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