Matches in SemOpenAlex for { <https://semopenalex.org/work/W2110603914> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2110603914 abstract "Light emitting diodes (LED), which are available as small monochromatic light sources with characteristic features such as maximum illumination power combined with minimum energy consumption and extremely long lifespan have already proved as a highly potential low-cost alternative for specific diagnostic applications in clinical medicine such as tuberculosis fluorescence microscopy. Likewise, the most reliable evaluation of Her-2/neu (c-erbB2) gene amplification, which has been established in the last few years for routine diagnosis in clinical pathology as determinant towards Herceptin-based treatment of patients with breast cancer, is based on fluorescence in situ hybridization (FISH) and corresponding high priced fluorescence equipment. In order to test the possibility to utilize the advantages of low-cost LED technology on FISH analysis of c-erbB2 gene expression for routine diagnostic purposes, the applicability of a standard bright field Carl Zeiss Axiostar Plus microscope equipped with a Fraen AFTER (Amplified Fluorescence by Transmitted Excitation of Radiation) LED Fluorescence Microscope Kit for the detection of Her-2/neu gene signals was compared to an advanced Nikon Eclipse 80i fluorescence microscope in combination with a conventional 100W mercury vapor lamp. Both microscopes were fitted with the same Quicam FAST CCD digital camera to unequivocally compare the quality of the captured images. C-erbB2 gene expression was analyzed in 30 different human tissue samples of primary invasive breast cancer, following formalin fixation and subsequent paraffin-embedding. The Her2/neu gene signals (green) were identifiable in the tumor cells in all cases and images of equal quality were captured under almost identical conditions by 480 nm (blue) LED module equipped standard Axiostar microscope as compared to conventional fluorescence microscopy. In this first attempt, these monochromatic LED elements proved in principle to be suitable for the detection of Her-2/neu gene expression by FISH. Thus, our own experiences emphasize the high potential of this technology to provide a serious alternative to conventional fluorescence microscopy in routine pathology; representing a sustainable technological progress, this low-cost technology will clearly give direction also to the growing field of molecular pathology." @default.
- W2110603914 created "2016-06-24" @default.
- W2110603914 creator A5006365540 @default.
- W2110603914 creator A5040217995 @default.
- W2110603914 creator A5050065329 @default.
- W2110603914 creator A5051429857 @default.
- W2110603914 creator A5071498197 @default.
- W2110603914 creator A5074120700 @default.
- W2110603914 creator A5088582087 @default.
- W2110603914 date "2008-12-01" @default.
- W2110603914 modified "2023-10-09" @default.
- W2110603914 title "LED-FISH: Fluorescence microscopy based on light emitting diodes for the molecular analysis of Her-2/neu oncogene amplification" @default.
- W2110603914 cites W1970341069 @default.
- W2110603914 cites W2061005277 @default.
- W2110603914 cites W2075985888 @default.
- W2110603914 cites W2087924085 @default.
- W2110603914 cites W2115759102 @default.
- W2110603914 cites W2142598239 @default.
- W2110603914 doi "https://doi.org/10.1186/1746-1596-3-49" @default.
- W2110603914 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2615417" @default.
- W2110603914 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19087271" @default.
- W2110603914 hasPublicationYear "2008" @default.
- W2110603914 type Work @default.
- W2110603914 sameAs 2110603914 @default.
- W2110603914 citedByCount "7" @default.
- W2110603914 countsByYear W21106039142012 @default.
- W2110603914 countsByYear W21106039142014 @default.
- W2110603914 countsByYear W21106039142015 @default.
- W2110603914 countsByYear W21106039142022 @default.
- W2110603914 crossrefType "journal-article" @default.
- W2110603914 hasAuthorship W2110603914A5006365540 @default.
- W2110603914 hasAuthorship W2110603914A5040217995 @default.
- W2110603914 hasAuthorship W2110603914A5050065329 @default.
- W2110603914 hasAuthorship W2110603914A5051429857 @default.
- W2110603914 hasAuthorship W2110603914A5071498197 @default.
- W2110603914 hasAuthorship W2110603914A5074120700 @default.
- W2110603914 hasAuthorship W2110603914A5088582087 @default.
- W2110603914 hasBestOaLocation W21106039141 @default.
- W2110603914 hasConcept C104317684 @default.
- W2110603914 hasConcept C120665830 @default.
- W2110603914 hasConcept C121332964 @default.
- W2110603914 hasConcept C142724271 @default.
- W2110603914 hasConcept C147080431 @default.
- W2110603914 hasConcept C169274487 @default.
- W2110603914 hasConcept C192562407 @default.
- W2110603914 hasConcept C2777542201 @default.
- W2110603914 hasConcept C30481170 @default.
- W2110603914 hasConcept C40833965 @default.
- W2110603914 hasConcept C54355233 @default.
- W2110603914 hasConcept C67649825 @default.
- W2110603914 hasConcept C71924100 @default.
- W2110603914 hasConcept C86803240 @default.
- W2110603914 hasConcept C91881484 @default.
- W2110603914 hasConceptScore W2110603914C104317684 @default.
- W2110603914 hasConceptScore W2110603914C120665830 @default.
- W2110603914 hasConceptScore W2110603914C121332964 @default.
- W2110603914 hasConceptScore W2110603914C142724271 @default.
- W2110603914 hasConceptScore W2110603914C147080431 @default.
- W2110603914 hasConceptScore W2110603914C169274487 @default.
- W2110603914 hasConceptScore W2110603914C192562407 @default.
- W2110603914 hasConceptScore W2110603914C2777542201 @default.
- W2110603914 hasConceptScore W2110603914C30481170 @default.
- W2110603914 hasConceptScore W2110603914C40833965 @default.
- W2110603914 hasConceptScore W2110603914C54355233 @default.
- W2110603914 hasConceptScore W2110603914C67649825 @default.
- W2110603914 hasConceptScore W2110603914C71924100 @default.
- W2110603914 hasConceptScore W2110603914C86803240 @default.
- W2110603914 hasConceptScore W2110603914C91881484 @default.
- W2110603914 hasIssue "1" @default.
- W2110603914 hasLocation W21106039141 @default.
- W2110603914 hasLocation W21106039142 @default.
- W2110603914 hasLocation W21106039143 @default.
- W2110603914 hasLocation W21106039144 @default.
- W2110603914 hasOpenAccess W2110603914 @default.
- W2110603914 hasPrimaryLocation W21106039141 @default.
- W2110603914 hasRelatedWork W2007423067 @default.
- W2110603914 hasRelatedWork W2029779669 @default.
- W2110603914 hasRelatedWork W2161433272 @default.
- W2110603914 hasRelatedWork W2315436027 @default.
- W2110603914 hasRelatedWork W2883259687 @default.
- W2110603914 hasRelatedWork W2894139091 @default.
- W2110603914 hasRelatedWork W4211107619 @default.
- W2110603914 hasRelatedWork W4248117623 @default.
- W2110603914 hasRelatedWork W4362562564 @default.
- W2110603914 hasRelatedWork W1637909 @default.
- W2110603914 hasVolume "3" @default.
- W2110603914 isParatext "false" @default.
- W2110603914 isRetracted "false" @default.
- W2110603914 magId "2110603914" @default.
- W2110603914 workType "article" @default.