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• W2110646721 endingPage "3526" @default.
• W2110646721 startingPage "3512" @default.
• W2110646721 abstract "Herpes simplex virus DNA replication proteins localize in characteristic patterns corresponding to viral DNA replication structures in the infected cell nucleus. The intranuclear spatial organization of the HSV DNA replication structures and the factors regulating their nuclear location remain to be defined. We have used the HSV ICP8 DNA-binding protein and bromodeoxyuridine labeling as markers for sites of herpesviral DNA synthesis to examine the spatial organization of these structures within the cell nucleus. Confocal microscopy and three-dimensional computer graphics reconstruction of optical series through infected cells indicated that viral DNA replication structures extend through the interior of the cell nucleus and appear to be spatially separate from the nuclear lamina. Examination of viral DNA replication structures in infected, binucleate cells showed similar or virtually identical patterns of DNA replication structures oriented along a twofold axis of symmetry between many of the sister nuclei. These results demonstrate that HSV DNA replication structures are organized in the interior of the nucleus and that their location is defined by preexisting host cell nuclear architecture, probably the internal nuclear matrix." @default.
• W2110646721 created "2016-06-24" @default.
• W2110646721 creator A5045054113 @default.
• W2110646721 creator A5067933844 @default.
• W2110646721 date "1994-06-01" @default.
• W2110646721 modified "2023-09-26" @default.
• W2110646721 title "Preexisting nuclear architecture defines the intranuclear location of herpesvirus DNA replication structures" @default.
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• W2110646721 doi "https://doi.org/10.1128/jvi.68.6.3512-3526.1994" @default.
• W2110646721 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/236855" @default.
• W2110646721 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8189490" @default.
• W2110646721 hasPublicationYear "1994" @default.
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