FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2110654860> ?p ?o ?g. }

• W2110654860 endingPage "489" @default.
• W2110654860 startingPage "480" @default.
• W2110654860 abstract "Helper-dependent, or gutted, adenoviruses (Ad) lack viral coding sequences, resulting in reduced immunotoxicity compared with conventional Ad vectors. Gutted Ad growth requires a conventional Ad to supply replication and packaging functions in trans. Methods that allow high-titer growth of gutted vectors while reducing helper contamination, and which use safer helper viruses, will facilitate the use of gutted Ad vectors in vivo.Replication-defective helper viruses were generated that are deleted for Ad E1, E2b and E3 genes, but which contain loxP sites flanking the packaging signal. Complementing Ad packaging cell lines (C7-cre cells) were also generated by transfecting 293 cells with the Ad E2b genes encoding DNA polymerase and pre-terminal protein, and with a cre-recombinase plasmid.We show that C7-cre cells allow efficient production of gutted Ad using deltaE1 + deltaE2b + deltaE3 helper viruses whose growth can be limited by cre-loxP-mediated excision of the packaging signal. Gutted Ad vectors carrying approximately 28 kb cassettes expressing full-length dystrophin were prepared at high titers, similar to those obtained with E2b+ helpers, with a resulting helper contamination of <1%.These new packaging cell lines and helper viruses offer several significant advantages for gutted Ad vector production. They allow gutted virus amplification using a reduced number of passages, which should reduce the chances of selecting rearranged products. Furthermore, the residual helper contamination in gutted vector preparations should be less able to elicit immunological reactions upon delivery to tissues, since E2b-deleted vectors display a profound reduction in viral gene expression." @default.
• W2110654860 created "2016-06-24" @default.
• W2110654860 creator A5008050442 @default.
• W2110654860 creator A5028738771 @default.
• W2110654860 creator A5043291137 @default.
• W2110654860 creator A5052317872 @default.
• W2110654860 creator A5080494386 @default.
• W2110654860 date "2002-06-20" @default.
• W2110654860 modified "2023-10-13" @default.
• W2110654860 title "Gutted adenoviral vector growth using E1/E2b/E3-deleted helper viruses" @default.
• W2110654860 cites W152758573 @default.
• W2110654860 cites W1531311762 @default.
• W2110654860 cites W1565284380 @default.
• W2110654860 cites W1565397798 @default.
• W2110654860 cites W1586130277 @default.
• W2110654860 cites W180023886 @default.
• W2110654860 cites W1967446284 @default.
• W2110654860 cites W1973905531 @default.
• W2110654860 cites W1974747739 @default.
• W2110654860 cites W1980890790 @default.
• W2110654860 cites W2004646325 @default.
• W2110654860 cites W2008260185 @default.
• W2110654860 cites W2011762615 @default.
• W2110654860 cites W2017961829 @default.
• W2110654860 cites W2025740124 @default.
• W2110654860 cites W2035600956 @default.
• W2110654860 cites W2044296969 @default.
• W2110654860 cites W2044629552 @default.
• W2110654860 cites W2069510338 @default.
• W2110654860 cites W2073371551 @default.
• W2110654860 cites W2075921029 @default.
• W2110654860 cites W2081228214 @default.
• W2110654860 cites W2081825650 @default.
• W2110654860 cites W2087080914 @default.
• W2110654860 cites W2106287195 @default.
• W2110654860 cites W2107021343 @default.
• W2110654860 cites W2114445152 @default.
• W2110654860 cites W2129484395 @default.
• W2110654860 cites W2144714494 @default.
• W2110654860 cites W2163046219 @default.
• W2110654860 cites W2163192616 @default.
• W2110654860 cites W2325878933 @default.
• W2110654860 cites W2326792847 @default.
• W2110654860 doi "https://doi.org/10.1002/jgm.305" @default.
• W2110654860 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12221641" @default.
• W2110654860 hasPublicationYear "2002" @default.
• W2110654860 type Work @default.
• W2110654860 sameAs 2110654860 @default.
• W2110654860 citedByCount "27" @default.
• W2110654860 countsByYear W21106548602014 @default.
• W2110654860 crossrefType "journal-article" @default.
• W2110654860 hasAuthorship W2110654860A5008050442 @default.
• W2110654860 hasAuthorship W2110654860A5028738771 @default.
• W2110654860 hasAuthorship W2110654860A5043291137 @default.
• W2110654860 hasAuthorship W2110654860A5052317872 @default.
• W2110654860 hasAuthorship W2110654860A5080494386 @default.
• W2110654860 hasBestOaLocation W21106548602 @default.
• W2110654860 hasConcept C104317684 @default.
• W2110654860 hasConcept C140704245 @default.
• W2110654860 hasConcept C144001008 @default.
• W2110654860 hasConcept C15152581 @default.
• W2110654860 hasConcept C153911025 @default.
• W2110654860 hasConcept C159047783 @default.
• W2110654860 hasConcept C22744801 @default.
• W2110654860 hasConcept C2522874641 @default.
• W2110654860 hasConcept C32470452 @default.
• W2110654860 hasConcept C40767141 @default.
• W2110654860 hasConcept C49105822 @default.
• W2110654860 hasConcept C54355233 @default.
• W2110654860 hasConcept C55493867 @default.
• W2110654860 hasConcept C70721500 @default.
• W2110654860 hasConcept C86803240 @default.
• W2110654860 hasConcept C92087593 @default.
• W2110654860 hasConceptScore W2110654860C104317684 @default.
• W2110654860 hasConceptScore W2110654860C140704245 @default.
• W2110654860 hasConceptScore W2110654860C144001008 @default.
• W2110654860 hasConceptScore W2110654860C15152581 @default.
• W2110654860 hasConceptScore W2110654860C153911025 @default.
• W2110654860 hasConceptScore W2110654860C159047783 @default.
• W2110654860 hasConceptScore W2110654860C22744801 @default.
• W2110654860 hasConceptScore W2110654860C2522874641 @default.
• W2110654860 hasConceptScore W2110654860C32470452 @default.
• W2110654860 hasConceptScore W2110654860C40767141 @default.
• W2110654860 hasConceptScore W2110654860C49105822 @default.
• W2110654860 hasConceptScore W2110654860C54355233 @default.
• W2110654860 hasConceptScore W2110654860C55493867 @default.
• W2110654860 hasConceptScore W2110654860C70721500 @default.
• W2110654860 hasConceptScore W2110654860C86803240 @default.
• W2110654860 hasConceptScore W2110654860C92087593 @default.
• W2110654860 hasIssue "5" @default.
• W2110654860 hasLocation W21106548601 @default.
• W2110654860 hasLocation W21106548602 @default.
• W2110654860 hasLocation W21106548603 @default.
• W2110654860 hasOpenAccess W2110654860 @default.
• W2110654860 hasPrimaryLocation W21106548601 @default.
• W2110654860 hasRelatedWork W1981596422 @default.
• W2110654860 hasRelatedWork W1997582694 @default.
• W2110654860 hasRelatedWork W2011293012 @default.

• next