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- W2110679964 abstract "Evaluation of the acrosome reaction can shed light on the fertilising competence of spermatozoa. To eliminate false-positive results when evaluating the acrosome status of human sperm cells, two viability probes propidium iodide (PI) and 7-amino-actinomycin D (7-AAD) were compared for their ability to stain nonviable cells post-fixation and permeabilisation. Both the mean fluorescence and % dead cells differed significantly with time (P < 0.0001). Unlike PI, 7-AAD did not leach from cells and fluorescence remained stable for up to 4 h. Furthermore, 7-AAD proved to be a proficient marker to exclude dead sperm cells during flow cytometric evaluation of ionophore-induced acrosome reaction." @default.
- W2110679964 created "2016-06-24" @default.
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- W2110679964 date "2010-02-01" @default.
- W2110679964 modified "2023-10-17" @default.
- W2110679964 title "Comparison between propidium iodide and 7-amino-actinomycin-D for viability assessment during flow cytometric analyses of the human sperm acrosome" @default.
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- W2110679964 doi "https://doi.org/10.1111/j.1439-0272.2009.00949.x" @default.
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