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- W2110747452 abstract "ABSTRACT The spx gene of Bacillus subtilis encodes a global regulator that controls transcription initiation in response to oxidative stress by interaction with RNA polymerase (RNAP). It is located in a dicistronic operon with the yjbC gene. The spx gene DNA complements an spx null mutation with respect to disulfide stress resistance, suggesting that spx is transcribed from a promoter located in the intergenic region of yjbC and spx . Transcription of the yjbC-spx operon has been reported to be driven by four promoters, three (P 1 , P 2 , and P B ) residing upstream of yjbC and one (P M ) located in the intergenic region between yjbC and spx . Primer extension analysis uncovered a second intergenic promoter, P 3 , from which transcription is elevated in cells treated with the thiol-specific oxidant diamide. P 3 is utilized by the σ A form of RNA polymerase in vitro without the involvement of a transcriptional activator. Transcriptional induction from P 3 did not require an Spx-RNAP interaction and was observed in a deletion mutant lacking DNA upstream of position −40 of the P 3 promoter start site. Deletion mutants with endpoints 3′ to the P 3 transcriptional start site (positions +5, +15, and +30) showed near-constitutive transcription at the induced level, indicating the presence of a negative control element downstream of the P 3 promoter sequence. Point mutations characterized by bgaB fusion expression and primer extension analyses uncovered evidence for a second cis -acting site in the P 3 promoter sequence itself. The data indicate that spx transcription is under negative transcriptional control that is reversed when disulfide stress is encountered." @default.
- W2110747452 created "2016-06-24" @default.
- W2110747452 creator A5055692290 @default.
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- W2110747452 date "2007-03-01" @default.
- W2110747452 modified "2023-10-18" @default.
- W2110747452 title "Transcription from the P <sub>3</sub> Promoter of the <i>Bacillus subtilis spx</i> Gene Is Induced in Response to Disulfide Stress" @default.
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- W2110747452 doi "https://doi.org/10.1128/jb.01519-06" @default.
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