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• W2110777780 endingPage "e47530" @default.
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• W2110777780 abstract "Most microorganisms in nature are uncultured with unknown functionality. Sequence-based metagenomics alone answers 'who/what are there?' but not 'what are they doing and who is doing it and how?'. Function-based metagenomics reveals gene function but is usually limited by the specificity and sensitivity of screening strategies, especially the identification of clones whose functional gene expression has no distinguishable activity or phenotypes. A 'biosensor-based genetic transducer' (BGT) technique, which employs a whole-cell biosensor to quantitatively detect expression of inserted genes encoding designated functions, is able to screen for functionality of unknown genes from uncultured microorganisms. In this study, BGT was integrated with Stable isotope probing (SIP)-enabled Metagenomics to form a culture-independent SMB toolbox. The utility of this approach was demonstrated in the discovery of a novel functional gene cluster in naphthalene contaminated groundwater. Specifically, metagenomic sequencing of the (13)C-DNA fraction obtained by SIP indicated that an uncultured Acidovorax sp. was the dominant key naphthalene degrader in-situ, although three culturable Pseudomonas sp. degraders were also present in the same groundwater. BGT verified the functionality of a new nag2 operon which co-existed with two other nag and two nah operons for naphthalene biodegradation in the same microbial community. Pyrosequencing analysis showed that the nag2 operon was the key functional operon in naphthalene degradation in-situ, and shared homology with both nag operons in Ralstonia sp. U2 and Polaromonas naphthalenivorans CJ2. The SMB toolbox will be useful in providing deep insights into uncultured microorganisms and unravelling their ecological roles in natural environments." @default.
• W2110777780 created "2016-06-24" @default.
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• W2110777780 date "2012-10-17" @default.
• W2110777780 modified "2023-09-27" @default.
• W2110777780 title "A Culture-Independent Approach to Unravel Uncultured Bacteria and Functional Genes in a Complex Microbial Community" @default.
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• W2110777780 doi "https://doi.org/10.1371/journal.pone.0047530" @default.
• W2110777780 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3474725" @default.
• W2110777780 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23082176" @default.
• W2110777780 hasPublicationYear "2012" @default.
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