FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2110837959> ?p ?o ?g. }

• W2110837959 endingPage "338" @default.
• W2110837959 startingPage "325" @default.
• W2110837959 abstract "Polychlorinated biphenyls (PCBs) are ubiquitous and persistent environmental contaminants that disturb normal endocrine functions, including gonadal functions in humans and mammals. In the present study, we examined the direct effects of PCB on rat Leydig cells in vitro . Adult Leydig cells were purified by Percoll gradient centrifugation method and the purity of Leydig cells was also determined by 3β-hydroxysteroid dehydrogenase (3β-HSD) staining method. Purified Leydig cells were exposed to different concentrations (10 − 10 –10 − 7 M) of PCB (Aroclor 1254) for 24 h under basal and LH-stimulated conditions. After the experimental period, cultured media were collected and used for the assay of testosterone and estradiol. The treated cells were used for the quantification of cell-surface LH receptors and activities of steroidogenic enzymes, such as cytochrome P 450 side-chain cleavage enzyme (P 450 scc), 3β-HSD, and 17β-hydroxysteroid dehydrogenase (17β-HSD). Leydig cellular enzymatic antioxidants, such as superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, γ-glutamyl transpeptidase, glutathione- S -transferase, and nonenzymatic antioxidants, such as vitamins C and E, were assayed. Lipid peroxidation (LPO) and reactive oxygen species (ROS) were also estimated in Leydig cells. In addition, total RNA was isolated from control and Aroclor 1254-exposed Leydig cells to monitor the steady-state mRNA levels by reverse transcription(RT)-PCR for steroidogenic acute-regulatory (StAR) protein, cytochrome P 450 scc, 3β-HSD, and 17β-HSD. Our results indicated that Aroclor 1254 (10 − 9 , 10 − 8 , and 10 − 7 M) treatments significantly inhibit basal and LH-stimulated testosterone and estradiol production. In addition, the activities of steroidogenic enzymes, enzymatic and nonenzymatic antioxidants were significantly diminished in a dose-dependent manner. However, LPO and ROS were elevated in a dose-dependent manner under basal and LH-stimulated conditions. RT-PCR analysis of StAR mRNA level showed a decrease only in 10 − 7 M dose of Aroclor 1254 treatment, while cytochrome P 450 scc, 3β-HSD, and 17β-HSD mRNAs were drastically decreased in both 10 − 8 and 10 − 7 M Aroclor 1254 treatment. These findings suggest that PCBs can act directly on Leydig cells to diminish testosterone production by inhibiting gene expression of steroidogenic enzymes and antioxidant system." @default.
• W2110837959 created "2016-06-24" @default.
• W2110837959 creator A5000105829 @default.
• W2110837959 creator A5025695931 @default.
• W2110837959 creator A5034444556 @default.
• W2110837959 creator A5051479809 @default.
• W2110837959 date "2007-02-01" @default.
• W2110837959 modified "2023-10-10" @default.
• W2110837959 title "Effects of polychlorinated biphenyl (Aroclor 1254) on steroidogenesis and antioxidant system in cultured adult rat Leydig cells" @default.
• W2110837959 cites W1487193306 @default.
• W2110837959 cites W1529656635 @default.
• W2110837959 cites W1547664885 @default.
• W2110837959 cites W1559601107 @default.
• W2110837959 cites W1581414347 @default.
• W2110837959 cites W1610204701 @default.
• W2110837959 cites W1775749144 @default.
• W2110837959 cites W1899191701 @default.
• W2110837959 cites W1963885414 @default.
• W2110837959 cites W1964936940 @default.
• W2110837959 cites W1968900944 @default.
• W2110837959 cites W1970859659 @default.
• W2110837959 cites W1979286570 @default.
• W2110837959 cites W1982144156 @default.
• W2110837959 cites W1985690685 @default.
• W2110837959 cites W1988085199 @default.
• W2110837959 cites W1988729104 @default.
• W2110837959 cites W1990247412 @default.
• W2110837959 cites W1990284973 @default.
• W2110837959 cites W1990554612 @default.
• W2110837959 cites W1991072417 @default.
• W2110837959 cites W1994053051 @default.
• W2110837959 cites W1994702338 @default.
• W2110837959 cites W1999395281 @default.
• W2110837959 cites W2002116196 @default.
• W2110837959 cites W2002337702 @default.
• W2110837959 cites W2006182150 @default.
• W2110837959 cites W2008532902 @default.
• W2110837959 cites W2010523646 @default.
• W2110837959 cites W2016848752 @default.
• W2110837959 cites W2017125567 @default.
• W2110837959 cites W2026252851 @default.
• W2110837959 cites W2043366157 @default.
• W2110837959 cites W2044721113 @default.
• W2110837959 cites W2046166649 @default.
• W2110837959 cites W2050951459 @default.
• W2110837959 cites W2051448662 @default.
• W2110837959 cites W2060194897 @default.
• W2110837959 cites W2060556958 @default.
• W2110837959 cites W2063795296 @default.
• W2110837959 cites W2069513152 @default.
• W2110837959 cites W2078683373 @default.
• W2110837959 cites W2081337319 @default.
• W2110837959 cites W2082589589 @default.
• W2110837959 cites W2088182597 @default.
• W2110837959 cites W2088866343 @default.
• W2110837959 cites W2089618682 @default.
• W2110837959 cites W2091308342 @default.
• W2110837959 cites W2092963288 @default.
• W2110837959 cites W2093631184 @default.
• W2110837959 cites W2093787853 @default.
• W2110837959 cites W2108146842 @default.
• W2110837959 cites W2119646277 @default.
• W2110837959 cites W2132547534 @default.
• W2110837959 cites W2138484830 @default.
• W2110837959 cites W2147322409 @default.
• W2110837959 cites W2149380741 @default.
• W2110837959 cites W2153549032 @default.
• W2110837959 cites W2158237570 @default.
• W2110837959 cites W2160677773 @default.
• W2110837959 cites W2169105311 @default.
• W2110837959 cites W2336083422 @default.
• W2110837959 cites W2411898170 @default.
• W2110837959 cites W2415460162 @default.
• W2110837959 cites W2523848082 @default.
• W2110837959 doi "https://doi.org/10.1677/joe.1.06874" @default.
• W2110837959 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17283232" @default.
• W2110837959 hasPublicationYear "2007" @default.
• W2110837959 type Work @default.
• W2110837959 sameAs 2110837959 @default.
• W2110837959 citedByCount "70" @default.
• W2110837959 countsByYear W21108379592012 @default.
• W2110837959 countsByYear W21108379592013 @default.
• W2110837959 countsByYear W21108379592014 @default.
• W2110837959 countsByYear W21108379592015 @default.
• W2110837959 countsByYear W21108379592016 @default.
• W2110837959 countsByYear W21108379592017 @default.
• W2110837959 countsByYear W21108379592018 @default.
• W2110837959 countsByYear W21108379592019 @default.
• W2110837959 countsByYear W21108379592020 @default.
• W2110837959 countsByYear W21108379592021 @default.
• W2110837959 countsByYear W21108379592022 @default.
• W2110837959 countsByYear W21108379592023 @default.
• W2110837959 crossrefType "journal-article" @default.
• W2110837959 hasAuthorship W2110837959A5000105829 @default.
• W2110837959 hasAuthorship W2110837959A5025695931 @default.
• W2110837959 hasAuthorship W2110837959A5034444556 @default.
• W2110837959 hasAuthorship W2110837959A5051479809 @default.
• W2110837959 hasBestOaLocation W21108379591 @default.

• next