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• W2110873152 endingPage "358" @default.
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• W2110873152 abstract "Signal peptide peptidase A (SppA) is a membrane-bound self-compartmentalized serine protease that functions to cleave the remnant signal peptides left behind after protein secretion and cleavage by signal peptidases. SppA is found in plants, archaea and bacteria. Here, we report the first crystal structure of a Gram-positive bacterial SppA. The 2.4-Å-resolution structure of Bacillus subtilis SppA (SppABS) catalytic domain reveals eight SppABS molecules in the asymmetric unit, forming a dome-shaped octameric complex. The octameric state of SppABS is supported by analytical size-exclusion chromatography and multi-angle light scattering analysis. Our sequence analysis, mutagenesis and activity assays are consistent with Ser147 serving as the nucleophile and Lys199 serving as the general base; however, they are located in different region of the protein, more than 29 Å apart. Only upon assembling the octamer do the serine and lysine come within close proximity, with neighboring protomers each providing one-half of the catalytic dyad, thus producing eight separate active sites within the complex, twice the number seen within Escherichia coli SppA (SppAEC). The SppABS S1 substrate specificity pocket is deep, narrow and hydrophobic, but with a polar bottom. The S3 pocket, which is constructed from two neighboring proteins, is shallower, wider and more polar than the S1 pocket. A comparison of these pockets to those seen in SppAEC reveals a significant difference in the size and shape of the S1 pocket, which we show is reflected in the repertoire of peptides the enzymes are capable of cleaving." @default.
• W2110873152 created "2016-06-24" @default.
• W2110873152 creator A5023499823 @default.
• W2110873152 creator A5032034661 @default.
• W2110873152 creator A5048104268 @default.
• W2110873152 date "2012-06-01" @default.
• W2110873152 modified "2023-10-03" @default.
• W2110873152 title "Crystal Structure of Bacillus subtilis Signal Peptide Peptidase A" @default.
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• W2110873152 doi "https://doi.org/10.1016/j.jmb.2012.03.020" @default.
• W2110873152 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22472423" @default.
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