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• W2110947328 abstract "Abstract In humans with atopic dermatitis and in mouse models of IgE‐mediated allergic diseases, evidence is mounting that the stratum corneum (SC) provides an important barrier against environmental allergens. At this time, it is not known whether the SC has a similar role in dogs, especially in those with atopic dermatitis. The objectives of this pilot study were to determine whether SC removal led to earlier and stronger sensitization of atopic dogs to Dermatophagoides farinae (Df) house dust mites. Five Maltese‐beagle atopic (MBA) dogs were sensitized epicutaneously after the SC was removed with ten tape strips (TS group), while sensitization was done without tape strips in five other MBA dogs (nontape stripping; NTS group). During this 16 week study, sensitization was assessed with allergen‐specific IgE serology, intradermal testing with Df allergens and determination of stimulation indices of blood mononuclear cells cultured with Df and stained for CD4 and the activation markers CD25 or CD30. Compared with dogs from the NTS group, those of the TS group exhibited earlier rises in Df‐specific IgE serum levels, usually had higher allergen‐specific IgE titres, showed higher intradermal test reactivity and had earlier increases and higher percentages of CD25‐ or CD30‐positive activated allergen‐specific peripheral CD4‐positive T lymphocytes. These observations implicate a role of the SC as a barrier limiting sensitization to exogenous allergens in this experimental atopic dog model." @default.
• W2110947328 created "2016-06-24" @default.
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• W2110947328 date "2010-12-09" @default.
• W2110947328 modified "2023-10-16" @default.
• W2110947328 title "Stratum corneum removal facilitates experimental sensitization to mite allergens in atopic dogs" @default.
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• W2110947328 doi "https://doi.org/10.1111/j.1365-3164.2010.00938.x" @default.
• W2110947328 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21143539" @default.
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