FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2110972562> ?p ?o ?g. }

• W2110972562 endingPage "e111487" @default.
• W2110972562 startingPage "e111487" @default.
• W2110972562 abstract "TNF-alpha-related-apoptosis-inducing-ligand (TRAIL) has been explored as a therapeutic drug to kill cancer cells. Cancer cells in the circulation are subjected to apoptosis-inducing factors. Despite the presence of these factors, cells are able to extravasate and metastasize. The homotypic and heterotypic cell-cell interactions in a tumor are known to play a crucial role in bestowing important characteristics to cancer cells that leave the primary site. Spheroid cell culture has been extensively used to mimic these physiologically relevant interactions. In this work, we show that the breast cancer cell lines BT20 and MCF7, cultured as 3D tumor spheroids, are more resistant to TRAIL-mediated apoptosis by downregulating the expression of death receptors (DR4 and DR5) that initiate TRAIL-mediated apoptosis. For comparison, we also investigated the effect of TRAIL on cells cultured as a 2D monolayer. Our results indicate that tumor spheroids are enriched for CD44hiCD24loALDH1hi cells, a phenotype that is predominantly known to be a marker for breast cancer stem cells. Furthermore, we attribute the TRAIL-resistance and cancer stem cell phenotype observed in tumor spheroids to the upregulation of cyclooxygenase-2 (COX-2)/prostaglandin E2 (PGE₂) pathway. We show that inhibition of the COX-2/PGE₂ pathway by treating tumor spheroids with NS-398, a selective COX-2 inhibitor, reverses the TRAIL-resistance and decreases the incidence of a CD44hiCD24lo population. Additionally, we show that siRNA mediated knockdown of COX-2 expression in MCF7 cells render them sensitive to TRAIL by increasing the expression of DR4 and DR5. Collectively, our results show the effect of the third-dimension on the response of breast cancer cells to TRAIL and suggest a therapeutic target to overcome TRAIL-resistance." @default.
• W2110972562 created "2016-06-24" @default.
• W2110972562 creator A5001182846 @default.
• W2110972562 creator A5010317825 @default.
• W2110972562 creator A5077360138 @default.
• W2110972562 creator A5081005464 @default.
• W2110972562 creator A5085492763 @default.
• W2110972562 date "2014-10-24" @default.
• W2110972562 modified "2023-10-14" @default.
• W2110972562 title "TRAIL-Mediated Apoptosis in Breast Cancer Cells Cultured as 3D Spheroids" @default.
• W2110972562 cites W122518273 @default.
• W2110972562 cites W1594540235 @default.
• W2110972562 cites W1967992429 @default.
• W2110972562 cites W1986130693 @default.
• W2110972562 cites W1986713431 @default.
• W2110972562 cites W2008772139 @default.
• W2110972562 cites W2008854828 @default.
• W2110972562 cites W2014713088 @default.
• W2110972562 cites W2025534438 @default.
• W2110972562 cites W2029572050 @default.
• W2110972562 cites W2034071655 @default.
• W2110972562 cites W2036677192 @default.
• W2110972562 cites W2042222655 @default.
• W2110972562 cites W2043904711 @default.
• W2110972562 cites W2045255643 @default.
• W2110972562 cites W2045477445 @default.
• W2110972562 cites W2045915548 @default.
• W2110972562 cites W2051522429 @default.
• W2110972562 cites W2059558094 @default.
• W2110972562 cites W2062069198 @default.
• W2110972562 cites W2070291618 @default.
• W2110972562 cites W2072758361 @default.
• W2110972562 cites W2073319565 @default.
• W2110972562 cites W2078352032 @default.
• W2110972562 cites W2088291142 @default.
• W2110972562 cites W2088481746 @default.
• W2110972562 cites W2090195784 @default.
• W2110972562 cites W2094570524 @default.
• W2110972562 cites W2098509343 @default.
• W2110972562 cites W2102085111 @default.
• W2110972562 cites W2102646523 @default.
• W2110972562 cites W2111034566 @default.
• W2110972562 cites W2117476538 @default.
• W2110972562 cites W2117692326 @default.
• W2110972562 cites W2125439393 @default.
• W2110972562 cites W2129883523 @default.
• W2110972562 cites W2131706926 @default.
• W2110972562 cites W2136445803 @default.
• W2110972562 cites W2137586381 @default.
• W2110972562 cites W2142416414 @default.
• W2110972562 cites W2144642824 @default.
• W2110972562 cites W2148572497 @default.
• W2110972562 cites W2151136390 @default.
• W2110972562 cites W2152064969 @default.
• W2110972562 cites W2155412969 @default.
• W2110972562 cites W2156222101 @default.
• W2110972562 cites W2161390424 @default.
• W2110972562 cites W2163642722 @default.
• W2110972562 cites W2164899193 @default.
• W2110972562 cites W2166339039 @default.
• W2110972562 cites W2167172107 @default.
• W2110972562 cites W2330124130 @default.
• W2110972562 doi "https://doi.org/10.1371/journal.pone.0111487" @default.
• W2110972562 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4208846" @default.
• W2110972562 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25343626" @default.
• W2110972562 hasPublicationYear "2014" @default.
• W2110972562 type Work @default.
• W2110972562 sameAs 2110972562 @default.
• W2110972562 citedByCount "38" @default.
• W2110972562 countsByYear W21109725622015 @default.
• W2110972562 countsByYear W21109725622016 @default.
• W2110972562 countsByYear W21109725622017 @default.
• W2110972562 countsByYear W21109725622018 @default.
• W2110972562 countsByYear W21109725622019 @default.
• W2110972562 countsByYear W21109725622020 @default.
• W2110972562 countsByYear W21109725622021 @default.
• W2110972562 countsByYear W21109725622022 @default.
• W2110972562 countsByYear W21109725622023 @default.
• W2110972562 crossrefType "journal-article" @default.
• W2110972562 hasAuthorship W2110972562A5001182846 @default.
• W2110972562 hasAuthorship W2110972562A5010317825 @default.
• W2110972562 hasAuthorship W2110972562A5077360138 @default.
• W2110972562 hasAuthorship W2110972562A5081005464 @default.
• W2110972562 hasAuthorship W2110972562A5085492763 @default.
• W2110972562 hasBestOaLocation W21109725621 @default.
• W2110972562 hasConcept C121608353 @default.
• W2110972562 hasConcept C1491633281 @default.
• W2110972562 hasConcept C175369904 @default.
• W2110972562 hasConcept C185592680 @default.
• W2110972562 hasConcept C190283241 @default.
• W2110972562 hasConcept C28328180 @default.
• W2110972562 hasConcept C2908647359 @default.
• W2110972562 hasConcept C31573885 @default.
• W2110972562 hasConcept C502942594 @default.
• W2110972562 hasConcept C54355233 @default.
• W2110972562 hasConcept C55427017 @default.
• W2110972562 hasConcept C55493867 @default.
• W2110972562 hasConcept C71924100 @default.

• next