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- W2110973910 abstract "Abstract Structural characterization of arabinoxylans from wheat by matrix‐assisted laser desorption/ionization time‐of‐flight (MALDI‐TOF) and electrospray ionization (ESI) mass spectrometry using a Q‐TOF mass analyser (ESI‐Q‐TOF) or an ion trap (IT) mass analyser is presented. An arabinoxylan sample digested with endoxylanase A was analysed using MALDI‐TOF mass spectrometry (MS), resulting in the identification of molecular ions for structures with up to 22 monosaccharide residues. As the two‐component monosaccharides xylose and arabinose are isobaric, structures differing in the number of arabinose branching residues were indistinguishable based on molecular mass and also fragmentation pattern upon collision‐induced dissociation (CID). Permethylation followed by ESI‐CID analyses using ITMS was performed to obtain structural information regarding the number of arabinose branching residues and their spatial arrangement along the xylose backbone. Analysis of the signal corresponding to an oligomer with six monosaccharide residues showed the presence of at least four isomeric structures differing in degree of branching and position of the branched residue relative to the cleavage site of the enzyme. This is the first demonstration of the use of ESI‐ITMS for the structural characterization of arabinoxylan mixtures. Copyright © 2003 John Wiley & Sons, Ltd." @default.
- W2110973910 created "2016-06-24" @default.
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- W2110973910 date "2003-03-07" @default.
- W2110973910 modified "2023-10-10" @default.
- W2110973910 title "Characterization of plant oligosaccharides by matrix-assisted laser desorption/ionization and electrospray mass spectrometry" @default.
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- W2110973910 doi "https://doi.org/10.1002/jms.455" @default.
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