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- W2111124524 abstract "Abstract Unmodified and GRGDS peptide‐modified six arm PEG star based hydrogels (Star PEG) have been applied as a multifunctional, easy to handle coating system for textile polyvinylidene fluoride (PVDF) structures, which prevent unspecific protein and cell adsorption and control‐specific cell adhesion. The reactive isocyanate‐terminated Star PEG has been successfully applied to ammonia‐plasma treated two‐ and three‐dimensional PVDF surfaces. Easy modification of the surface hydrogel by mixing in of GRGDS peptide during the coating step or subsequent coupling of GRGDS was determined by TOF‐SIMS. Unmodified and GRGDS‐functionalized hydrogel surfaces show distinct protein repellency, as demonstrated by fluorescence microscopy after incubation with fluorescent labeled proteins and Surface MALDI‐TOF‐Mass Spectroscopy. Cell culture experiments with primary human dermal fibroblasts, primary fetal rat fibroblasts, and human osteoblasts on GRGDS and/or KRSR Star PEG‐modified two‐ and three‐dimensional substrates show advancement in cell adhesion and proliferation compared with untreated PVDF surfaces, whereas pure star PEG‐coated surfaces show no cell adhesion. The combination of protein and cell repellent properties with specific biofunctionality and easy application of the coatings will enable their application for 3D‐scaffolds. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010" @default.
- W2111124524 created "2016-06-24" @default.
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- W2111124524 date "2009-05-08" @default.
- W2111124524 modified "2023-10-12" @default.
- W2111124524 title "Bio-functionalized star PEG-coated PVDF surfaces for cytocompatibility-improved implant components" @default.
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- W2111124524 doi "https://doi.org/10.1002/jbm.a.32478" @default.
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