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- W2111154300 abstract "RpoS, an alternative sigma factor produced by many Gram-negative bacteria, primarily controls genes that are expressed in stationary phase in response to nutrient deprivation. To test the idea that induction of RpoS in the exponential phase, when RpoS is not normally expressed, increases RpoS-dependent gene expression, we constructed a plasmid carrying the rpoS gene under the control of an IPTG (isopropyl-β-D-thiogalactopyranoside)-inducible T7lac promoter. Northern and Western analyses revealed that levels of RpoS mRNA and protein, respectively, increased in response to the inducer IPTG. Assays of changes in RpoS-dependent functions (catalase activity and glycogen accumulation), confirmed that induced RpoS was functional in exponential phase and was sufficient for the expression of RpoS-dependent functions. Controlled expression of RpoS and RpoS-dependent genes by plasmid-encoded rpoS may thus offer a useful tool for the study of RpoS-dependent gene expression.Key words: RpoS, regulon, gene expression, Escherichia coli." @default.
- W2111154300 created "2016-06-24" @default.
- W2111154300 creator A5034570880 @default.
- W2111154300 creator A5035603677 @default.
- W2111154300 date "2003-12-01" @default.
- W2111154300 modified "2023-09-24" @default.
- W2111154300 title "Controlled induction of the RpoS regulon inEscherichia coli, using an RpoS-expressing plasmid" @default.
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- W2111154300 doi "https://doi.org/10.1139/w03-096" @default.
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