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- W2111194219 abstract "Glucagon-storing cells were detected in the pancreatic islets of horse, guinea pig, rabbit, rat, dog and man by means of indirect immuno-histochemical procedures using fluorescein or peroxidase as markers of antiglucagon sera. By subsequently staining horse islets with selective staining techniques for A, B or D cells, it was directly ascertained that only A cells reacted to antiglucagon sera. A comparison of methods known to stain a granules with immuno-histochemical methods, as well asin vitro experiments on natural and synthetic glucagon, showed that the xanthydrol method, and perhaps also theo-phthalaldehyde method, should be regarded as specific histochemical tests for glucagon whereas the HCl-basic dye technique and phosphotungstic haematein should be considered as unspecific glucagon-staining methods,and Grimelius' silver as a method likely to be unrelated to glucagon. In ultrastructural investigations on the human pancreas, the core of the a granule heavily reacted to phosphotungstic acid and peroxidase-marked antiglucagon antibodies, while being unreactive to Grimelius' silver; conversely, the peripheral halo of theα granule heavily reacted to Grimelius' silver, while being poorly reactive or unreactive to phosphotungstic acid and anti-glucagon antibodies. Thus, both light and electron microscopy findings point to a structural and chemical hetereogeneity of theα granule." @default.
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- W2111194219 date "1971-06-01" @default.
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- W2111194219 title "Histochemical and ultrastructural studies on pancreatic A cells. Evidence for glucagon and non-glucagon components of the ? granule" @default.
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- W2111194219 doi "https://doi.org/10.1007/bf01212551" @default.
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