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• W2111200178 abstract "ABSTRACT The heat shock protein DnaK is essential for intramacrophagic replication of Brucella suis . The replacement of the stress-inducible, native dnaK promoter of B. suis by the promoter of the constitutively expressed bla gene resulted in temperature-independent synthesis of DnaK. In contrast to a dnaK null mutant, this strain grew at 37°C, with a thermal cutoff at 39°C. However, the constitutive dnaK mutant, which showed high sensitivity to H 2 O 2 -mediated stress, failed to multiply in murine macrophage-like cells and was rapidly eliminated in a mouse model of infection, adding strong arguments to our hypothesis that stress-mediated and heat shock promoter-dependent induction of dnaK is a crucial event in the intracellular replication of B. suis ." @default.
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• W2111200178 date "2002-03-01" @default.
• W2111200178 modified "2023-09-23" @default.
• W2111200178 title "Induction of <i>dnaK</i> through Its Native Heat Shock Promoter Is Necessary for Intramacrophagic Replication of <i>Brucella suis</i>" @default.
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• W2111200178 doi "https://doi.org/10.1128/iai.70.3.1631-1634.2002" @default.
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