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• W2111408770 abstract "Using a CD4-capture immunoassay for gp120, several strains of human immunodeficiency virus type 1 (HIV-1) grown in CD4-expressing T lymphoblastoid cells were found to contain little CD4-reactive gp120 (0.3-1.0 ng/ml) relative to virus titre (10(3.2)-10(5.0) TCID50/ml) and p24 antigen (80-1000 ng/ml). The measured CD4-reactive gp120 concentrations of HIV-1 suspensions grown in CD4-negative human neuroblastoma cells were 100- to 10,000-fold greater than those of HIV-1 grown in CD4-positive lymphoblastoid cells, even though both virus suspensions contained abundant viral gp120 as shown by immunoblot assay. It was postulated that CD4 derived from host cells might be associated with virions, concealing the binding domains of gp120. CD4 association with HIV-1 virions grown in CD4-positive cells was demonstrated directly by immunoblot assay of sucrose gradient-purified virus suspensions and by specific co-sedimentation of 125I-labelled OKT4 with virions propagated in CD4-expressing cells. CD4 coating of primary HIV-1 isolates grown in peripheral blood mononuclear cells was also observed. The biological significance of CD4 coating of HIV particles remains to be determined." @default.
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• W2111408770 date "1996-09-01" @default.
• W2111408770 modified "2023-10-18" @default.
• W2111408770 title "Human Immunodeficiency Virus Grown in CD4-Expressing Cells is Associated with CD4" @default.
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• W2111408770 doi "https://doi.org/10.1099/0022-1317-77-9-2015" @default.
• W2111408770 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8810998" @default.
• W2111408770 hasPublicationYear "1996" @default.
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