FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2111433181> ?p ?o ?g. }

• W2111433181 endingPage "511" @default.
• W2111433181 startingPage "497" @default.
• W2111433181 abstract "Epidermal-cell protoplasts from rye (Secale cereale L.) roots were voltage-clamped in both the whole-cell and outside-out membrane-patch modes. Time-dependent inwardly-rectified (IR) and outwardly-rectified (OR) K+-currents were recorded, as well as a ubiquitous, timeindependent (instantaneous) K+-current. The IR current activated at voltages more negative than —100mV with two exponentially rising components. The time-constant of the shorter component was voltage-independent, whereas the time-constant of the longer component was voltage-dependent, increasing as the activating voltage became more negative. The IR current showed no inactivation. The IR current deactivated with a single exponential timecourse. The steady-state IR current could be fitted to a Boltzmann function with —135 mV as the voltage at which the current was half-maximal and a minimal gating charge of 1.93. These parameters were insensitive to changes in EK. One component of the IR current was K + , but other ions were also permeable. The IR current was inhibited by extracellular Ca2+ , Ba2+ , Cs+, and TEA+, but was insensitive to quinine. Single channels with unitary conductances of 56 pS and 110 pS (in c.100 mM K+) were recorded at negative voltages. Two OR currents were observed. One had sigmoidal activation kinetics and activated at low positive voltages. The other activated more rapidly, with apparently exponential kinetics, at voltages 50–100 mV more positive than the first. Neither current showed inactivation and deactivation of OR currents followed a double exponential time-course. Unitary-conductances of the channels mediating these OR currents were 24 pS and 57 pS (in c.100 mM K+), respectively. Only the first type of OR current was studied in detail. This current activated with a sigmoidal time-course, which could be described using a Hodgkin-Huxley function with the activation variable raised to the second power. Its voltage-dependence was modulated in response to changes in EK and analysis of single-channel recordings indicated that the channel was K+-selective. The current was inhibited by Ba2+ and TEA+, but not Ca2+, Cs+ or quinine. The instantaneous current was selective for monovalent cations and K+ , Na+ and Cs+ were all permeant. It was inhibited by extracellular quinine and the instantaneous inward K+-current was reduced by extracellular Ca2+, Ba2+ and TEA+, as well as by competing permeant monovalent cations. The kinetics and pharmacology of these currents are compared with K+-currents across the plasma membrane of protoplasts from other root-derived cells and with K+ channels in the plasma membrane of rye roots studied following incorporation into artificial, planar lipid bilayers." @default.
• W2111433181 created "2016-06-24" @default.
• W2111433181 creator A5062554116 @default.
• W2111433181 creator A5081576425 @default.
• W2111433181 date "1995-01-01" @default.
• W2111433181 modified "2023-09-27" @default.
• W2111433181 title "Potassium currents across the plasma membrane of protoplasts derived from rye roots: a patch-clamp study" @default.
• W2111433181 cites W1533188319 @default.
• W2111433181 cites W1542649433 @default.
• W2111433181 cites W1556861444 @default.
• W2111433181 cites W1571541194 @default.
• W2111433181 cites W1771008006 @default.
• W2111433181 cites W1969078154 @default.
• W2111433181 cites W1981633711 @default.
• W2111433181 cites W1982369185 @default.
• W2111433181 cites W1983505512 @default.
• W2111433181 cites W1983934546 @default.
• W2111433181 cites W1996196738 @default.
• W2111433181 cites W1996299596 @default.
• W2111433181 cites W2009667219 @default.
• W2111433181 cites W2011211450 @default.
• W2111433181 cites W2012619013 @default.
• W2111433181 cites W2018004735 @default.
• W2111433181 cites W2021531638 @default.
• W2111433181 cites W202290981 @default.
• W2111433181 cites W2033136941 @default.
• W2111433181 cites W2034924339 @default.
• W2111433181 cites W2034942481 @default.
• W2111433181 cites W2035422711 @default.
• W2111433181 cites W2040106814 @default.
• W2111433181 cites W2046831855 @default.
• W2111433181 cites W2047614324 @default.
• W2111433181 cites W2048226662 @default.
• W2111433181 cites W2052804594 @default.
• W2111433181 cites W2078420488 @default.
• W2111433181 cites W2080699300 @default.
• W2111433181 cites W2084549735 @default.
• W2111433181 cites W2086605080 @default.
• W2111433181 cites W2087660299 @default.
• W2111433181 cites W2088526093 @default.
• W2111433181 cites W2109288052 @default.
• W2111433181 cites W2114482009 @default.
• W2111433181 cites W2117683643 @default.
• W2111433181 cites W2122792910 @default.
• W2111433181 cites W2135363210 @default.
• W2111433181 cites W2153691121 @default.
• W2111433181 cites W2154023394 @default.
• W2111433181 cites W2159709612 @default.
• W2111433181 cites W2398011604 @default.
• W2111433181 cites W3166379311 @default.
• W2111433181 cites W2034964262 @default.
• W2111433181 doi "https://doi.org/10.1093/jxb/46.5.497" @default.
• W2111433181 hasPublicationYear "1995" @default.
• W2111433181 type Work @default.
• W2111433181 sameAs 2111433181 @default.
• W2111433181 citedByCount "83" @default.
• W2111433181 countsByYear W21114331812012 @default.
• W2111433181 countsByYear W21114331812013 @default.
• W2111433181 countsByYear W21114331812014 @default.
• W2111433181 countsByYear W21114331812015 @default.
• W2111433181 countsByYear W21114331812016 @default.
• W2111433181 countsByYear W21114331812017 @default.
• W2111433181 countsByYear W21114331812018 @default.
• W2111433181 countsByYear W21114331812019 @default.
• W2111433181 countsByYear W21114331812021 @default.
• W2111433181 crossrefType "journal-article" @default.
• W2111433181 hasAuthorship W2111433181A5062554116 @default.
• W2111433181 hasAuthorship W2111433181A5081576425 @default.
• W2111433181 hasConcept C100837961 @default.
• W2111433181 hasConcept C113196181 @default.
• W2111433181 hasConcept C119599485 @default.
• W2111433181 hasConcept C121332964 @default.
• W2111433181 hasConcept C12554922 @default.
• W2111433181 hasConcept C127413603 @default.
• W2111433181 hasConcept C147789679 @default.
• W2111433181 hasConcept C147944092 @default.
• W2111433181 hasConcept C148043351 @default.
• W2111433181 hasConcept C148898269 @default.
• W2111433181 hasConcept C165801399 @default.
• W2111433181 hasConcept C170493617 @default.
• W2111433181 hasConcept C181911157 @default.
• W2111433181 hasConcept C185592680 @default.
• W2111433181 hasConcept C18903297 @default.
• W2111433181 hasConcept C194544171 @default.
• W2111433181 hasConcept C2778987702 @default.
• W2111433181 hasConcept C43617362 @default.
• W2111433181 hasConcept C55493867 @default.
• W2111433181 hasConcept C62520636 @default.
• W2111433181 hasConcept C81370116 @default.
• W2111433181 hasConcept C8171440 @default.
• W2111433181 hasConcept C86803240 @default.
• W2111433181 hasConcept C97355855 @default.
• W2111433181 hasConceptScore W2111433181C100837961 @default.
• W2111433181 hasConceptScore W2111433181C113196181 @default.
• W2111433181 hasConceptScore W2111433181C119599485 @default.
• W2111433181 hasConceptScore W2111433181C121332964 @default.
• W2111433181 hasConceptScore W2111433181C12554922 @default.
• W2111433181 hasConceptScore W2111433181C127413603 @default.

• next