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- W2111444253 abstract "Abstract The equilibrium and kinetic adsorption and desorption of bovine serum albumin (BSA), fraction V, on Sephadex A‐50 has been studied. The equilibrium data have been presented by a Langmuir isotherm for both adsorption and desorption conditions. The kinetics of adsorption in a batch system may be represented by a simple diffusional model. The kinetics of desorption of BSA in a batch system show a very fast initial rate due to the rapid shrinkage of the resin with changes in salt concentration. This has been modeled by including a protein exclusion term in the kinetics for desorption. The above information has been used to predict the breakthrough of BSA in a column operation. Under the pressure exerted in a column flow system the Sephadex A‐50 is severely compressed. The effect this has on the breakthrough rate of BSA has been demonstrated." @default.
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- W2111444253 date "1985-12-01" @default.
- W2111444253 modified "2023-10-16" @default.
- W2111444253 title "Prediction of adsorption and desorption of protein on dextran based ion-exchange resin" @default.
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- W2111444253 doi "https://doi.org/10.1002/aic.690311205" @default.
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