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- W2111446997 abstract "Polymers of adenylic and uridylic acids have been prepared from the monomers by the action of an enzyme isolated from the bacteria M. lysodéikticus. Polyadenylic acid has been found to undergo an interesting structural change in the vicinity of pH 6. This is reflected by a marked contraction of the molecule, as observed by light scattering and sedimentation, and a pronounced drop in ultraviolet extinction at λ = 257 mµ. The structural and optical transitions may be eliminated by formaldehyde treatment or by the addition of calcium ions. By direct titration it has been found that these effects reflect the titration of the adenine group and probably its subsequent electrostatic pairing with a negatively charged phosphate. The molecular interaction of polyadenylic and polyuridylic acids to form a stable complex has also been studied. It has been found that the interaction is blocked by: (1) the binding of a hydrogen ion by the 6-amino group of adenine, (2) formaldehyde blocking of the 6-amino group of adenine, and (3) ionization of the hydroxyl group of the pyrimidine ring of uridine." @default.
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- W2111446997 date "1958-07-01" @default.
- W2111446997 modified "2023-10-16" @default.
- W2111446997 title "Some properties of enzymatically produced polynucleotides" @default.
- W2111446997 doi "https://doi.org/10.1002/pol.1958.1203012103" @default.
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