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• W2111448629 abstract "Serratia marcescens has emerged in the last few years as an important nosocomial pathogen. Many methods for typing this organism have been described. In this study the random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was shown to be a convenient typing method for S. marcescens. Different combinations of primers previously used for typing other gram-negative bacilli were assessed. The combination of primer HLWL-74 and 1254 gave distinguishable patterns for different serotypes and proved to be the most satisfactory. By applying this combination to 175 isolates of S. marcescens, which could be classified into 38 groups on the basis of serotyping and phage typing, 73 different RAPD patterns with good reproducibility were obtained. This is, to our knowledge, the first application of the method to a large collection of S. rnarcescens representing a wide range of serotypes." @default.
• W2111448629 created "2016-06-24" @default.
• W2111448629 creator A5004014607 @default.
• W2111448629 creator A5016038192 @default.
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• W2111448629 date "1997-11-01" @default.
• W2111448629 modified "2023-10-03" @default.
• W2111448629 title "The use of RAPD-PCR as a typing method for Serratia marcescens" @default.
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• W2111448629 doi "https://doi.org/10.1099/00222615-46-11-913" @default.
• W2111448629 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9368531" @default.
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