FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2111497410> ?p ?o ?g. }

• W2111497410 endingPage "593" @default.
• W2111497410 startingPage "585" @default.
• W2111497410 abstract "In diploid cells of the yeast Saccharomyces cerevisiae, the α2 and a1 homeodomain proteins bind cooperatively to sites in the promoters of haploid cell-type-specific genes (hsg) to repress their expression. Although both proteins bind to the DNA, in the α2 homeodomain substitutions of residues that are involved in contacting the DNA have little or no effect on repression in vivo or cooperative DNA binding with a1 protein in vitro. This result brings up the question of the contribution of each protein in the heterodimer complex to the DNA-binding affinity and specificity. To determine the requirements for the a1-α2 homeodomain DNA recognition, we systematically introduced single base-pair substitutions in an a1-α2 DNA-binding site and examined their effects on repression in vivo and DNA binding in vitro. Our results show that nearly all substitutions that significantly decrease repression and DNA-binding affinity are at positions which are specifically contacted by either the α2 or a1 protein. Interestingly, an α2 mutant lacking side chains that make base-specific contacts in the major groove is able to discriminate between the wild-type and mutant DNA sites with the same sequence specificity as the wild-type protein. These results suggest that the specificity of α2 DNA binding in complex with a1 does not rely solely on the residues that make base-specific contacts. We have also examined the contribution of the a1 homeodomain to the binding affinity and specificity of the complex. In contrast to the lack of a defective phenotype produced by mutations in the α2 homeodomain, many of the alanine substitutions of residues in the a1 homeodomain have large effects on a1-α2-mediated repression and DNA binding. This result shows that the two proteins do not make equal contributions to the DNA-binding affinity of the complex." @default.
• W2111497410 created "2016-06-24" @default.
• W2111497410 creator A5013003121 @default.
• W2111497410 creator A5015374101 @default.
• W2111497410 creator A5066349760 @default.
• W2111497410 date "1999-01-01" @default.
• W2111497410 modified "2023-10-11" @default.
• W2111497410 title "The Yeast <b>a</b>1 and α2 Homeodomain Proteins Do Not Contribute Equally to Heterodimeric DNA Binding" @default.
• W2111497410 cites W1705407249 @default.
• W2111497410 cites W1788199927 @default.
• W2111497410 cites W1803509301 @default.
• W2111497410 cites W1925430508 @default.
• W2111497410 cites W1971170993 @default.
• W2111497410 cites W197372360 @default.
• W2111497410 cites W1975839628 @default.
• W2111497410 cites W1982910659 @default.
• W2111497410 cites W1986586522 @default.
• W2111497410 cites W1986748430 @default.
• W2111497410 cites W1994664042 @default.
• W2111497410 cites W1996045820 @default.
• W2111497410 cites W1996839811 @default.
• W2111497410 cites W2000669268 @default.
• W2111497410 cites W2004689349 @default.
• W2111497410 cites W2010108735 @default.
• W2111497410 cites W2012943750 @default.
• W2111497410 cites W2016812568 @default.
• W2111497410 cites W2017035463 @default.
• W2111497410 cites W2022987966 @default.
• W2111497410 cites W2039007871 @default.
• W2111497410 cites W2039478144 @default.
• W2111497410 cites W2043839008 @default.
• W2111497410 cites W2046278558 @default.
• W2111497410 cites W2047810769 @default.
• W2111497410 cites W2066424373 @default.
• W2111497410 cites W2076849893 @default.
• W2111497410 cites W2081346782 @default.
• W2111497410 cites W2085058883 @default.
• W2111497410 cites W2085418697 @default.
• W2111497410 cites W2088956217 @default.
• W2111497410 cites W2090654939 @default.
• W2111497410 cites W2108818873 @default.
• W2111497410 cites W2114618670 @default.
• W2111497410 cites W2123903336 @default.
• W2111497410 cites W2126448076 @default.
• W2111497410 cites W2133755882 @default.
• W2111497410 cites W2144784260 @default.
• W2111497410 cites W2212606280 @default.
• W2111497410 cites W2767273820 @default.
• W2111497410 cites W311690591 @default.
• W2111497410 cites W4254829742 @default.
• W2111497410 cites W99595223 @default.
• W2111497410 doi "https://doi.org/10.1128/mcb.19.1.585" @default.
• W2111497410 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/83916" @default.
• W2111497410 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9858582" @default.
• W2111497410 hasPublicationYear "1999" @default.
• W2111497410 type Work @default.
• W2111497410 sameAs 2111497410 @default.
• W2111497410 citedByCount "22" @default.
• W2111497410 countsByYear W21114974102017 @default.
• W2111497410 crossrefType "journal-article" @default.
• W2111497410 hasAuthorship W2111497410A5013003121 @default.
• W2111497410 hasAuthorship W2111497410A5015374101 @default.
• W2111497410 hasAuthorship W2111497410A5066349760 @default.
• W2111497410 hasBestOaLocation W21114974102 @default.
• W2111497410 hasConcept C101762097 @default.
• W2111497410 hasConcept C104317684 @default.
• W2111497410 hasConcept C107824862 @default.
• W2111497410 hasConcept C121587040 @default.
• W2111497410 hasConcept C150194340 @default.
• W2111497410 hasConcept C153911025 @default.
• W2111497410 hasConcept C185581394 @default.
• W2111497410 hasConcept C2777576037 @default.
• W2111497410 hasConcept C3662595 @default.
• W2111497410 hasConcept C5179208 @default.
• W2111497410 hasConcept C54355233 @default.
• W2111497410 hasConcept C552990157 @default.
• W2111497410 hasConcept C86339819 @default.
• W2111497410 hasConcept C86803240 @default.
• W2111497410 hasConcept C94966510 @default.
• W2111497410 hasConceptScore W2111497410C101762097 @default.
• W2111497410 hasConceptScore W2111497410C104317684 @default.
• W2111497410 hasConceptScore W2111497410C107824862 @default.
• W2111497410 hasConceptScore W2111497410C121587040 @default.
• W2111497410 hasConceptScore W2111497410C150194340 @default.
• W2111497410 hasConceptScore W2111497410C153911025 @default.
• W2111497410 hasConceptScore W2111497410C185581394 @default.
• W2111497410 hasConceptScore W2111497410C2777576037 @default.
• W2111497410 hasConceptScore W2111497410C3662595 @default.
• W2111497410 hasConceptScore W2111497410C5179208 @default.
• W2111497410 hasConceptScore W2111497410C54355233 @default.
• W2111497410 hasConceptScore W2111497410C552990157 @default.
• W2111497410 hasConceptScore W2111497410C86339819 @default.
• W2111497410 hasConceptScore W2111497410C86803240 @default.
• W2111497410 hasConceptScore W2111497410C94966510 @default.
• W2111497410 hasIssue "1" @default.
• W2111497410 hasLocation W21114974101 @default.
• W2111497410 hasLocation W21114974102 @default.
• W2111497410 hasLocation W21114974103 @default.

• next