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• W2111607906 endingPage "1695" @default.
• W2111607906 startingPage "1677" @default.
• W2111607906 abstract "Abstract Diterpene resin acids (DRAs) are specialized (secondary) metabolites of the oleoresin defense of conifers produced by diterpene synthases and cytochrome P450s of the CYP720B family. The evolution of DRA metabolism shares common origins with the biosynthesis of ent-kaurenoic acid, which is highly conserved in general (primary) metabolism of gibberellin biosynthesis. Transcriptome mining in species of spruce (Picea) and pine (Pinus) revealed CYP720Bs of four distinct clades. We cloned a comprehensive set of 12 different Sitka spruce (Picea sitchensis) CYP720Bs as full-length cDNAs. Spatial expression profiles, methyl jasmonate induction, and transcript enrichment in terpenoid-producing resin ducts suggested a role of CYP720B4 in DRA biosynthesis. CYP720B4 was characterized as a multisubstrate, multifunctional enzyme by the formation of oxygenated diterpenoids in metabolically engineered yeast, yeast in vivo transformation of diterpene substrates, in vitro assays with CYP720B4 protein produced in Escherichia coli, and alteration of DRA profiles in RNA interference-suppressed spruce seedlings. CYP720B4 was active with 24 different diterpenoid substrates, catalyzing consecutive C-18 oxidations in the biosynthesis of an array of diterpene alcohols, aldehydes, and acids. CYP720B4 was most active in the formation of dehydroabietic acid, a compound associated with insect resistance of Sitka spruce. We identified patterns of convergent evolution of CYP720B4 in DRA metabolism and ent-kaurene oxidase CYP701 in gibberellin metabolism and revealed differences in the evolution of specialized and general diterpene metabolism in a gymnosperm. The genomic and functional characterization of the gymnosperm CYP720B family highlights that the evolution of specialized metabolism involves substantial diversification relative to conserved, general metabolism." @default.
• W2111607906 created "2016-06-24" @default.
• W2111607906 creator A5007843032 @default.
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• W2111607906 creator A5071803745 @default.
• W2111607906 date "2011-10-12" @default.
• W2111607906 modified "2023-10-18" @default.
• W2111607906 title "Evolution of Diterpene Metabolism: Sitka Spruce CYP720B4 Catalyzes Multiple Oxidations in Resin Acid Biosynthesis of Conifer Defense against Insects " @default.
• W2111607906 cites W1541441004 @default.
• W2111607906 cites W1763753419 @default.
• W2111607906 cites W1951469402 @default.
• W2111607906 cites W1966217572 @default.
• W2111607906 cites W1971512481 @default.
• W2111607906 cites W1975165380 @default.
• W2111607906 cites W1985504443 @default.
• W2111607906 cites W1985615922 @default.
• W2111607906 cites W1986358152 @default.
• W2111607906 cites W1988907676 @default.
• W2111607906 cites W1992306108 @default.
• W2111607906 cites W1997119836 @default.
• W2111607906 cites W2003217244 @default.
• W2111607906 cites W2005601752 @default.
• W2111607906 cites W2006864838 @default.
• W2111607906 cites W2011353494 @default.
• W2111607906 cites W2013611909 @default.
• W2111607906 cites W2021842042 @default.
• W2111607906 cites W2022064793 @default.
• W2111607906 cites W2027887178 @default.
• W2111607906 cites W2029420003 @default.
• W2111607906 cites W2036148914 @default.
• W2111607906 cites W2037593399 @default.
• W2111607906 cites W2040237566 @default.
• W2111607906 cites W2042228810 @default.
• W2111607906 cites W2045860313 @default.
• W2111607906 cites W2061811810 @default.
• W2111607906 cites W2062281754 @default.
• W2111607906 cites W2065607696 @default.
• W2111607906 cites W2066687858 @default.
• W2111607906 cites W2068293364 @default.
• W2111607906 cites W2071099979 @default.
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• W2111607906 cites W2090068673 @default.
• W2111607906 cites W2092272370 @default.
• W2111607906 cites W2093893438 @default.
• W2111607906 cites W2094509873 @default.
• W2111607906 cites W2095625126 @default.
• W2111607906 cites W2096384646 @default.
• W2111607906 cites W2098093621 @default.
• W2111607906 cites W2098217287 @default.
• W2111607906 cites W2102005894 @default.
• W2111607906 cites W2103546861 @default.
• W2111607906 cites W2108478641 @default.
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• W2111607906 cites W2125160208 @default.
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• W2111607906 cites W2131211647 @default.
• W2111607906 cites W2136170436 @default.
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• W2111607906 cites W2138022567 @default.
• W2111607906 cites W2147171562 @default.
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• W2111607906 cites W2330580803 @default.
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• W2111607906 doi "https://doi.org/10.1104/pp.111.185843" @default.
• W2111607906 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3327196" @default.
• W2111607906 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21994349" @default.
• W2111607906 hasPublicationYear "2011" @default.
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