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• W2111625089 abstract "The operon nasACBH in Azotobacter vinelandii encodes nitrate and nitrite reductases that sequentially reduce nitrate to nitrite and to ammonium for nitrogen assimilation into organic molecules. Our previous analyses showed that nasACBH expression is subject to antitermination regulation that occurs upstream of the nasA gene in response to the availability of nitrate and nitrite. In this study, we continued expression analyses of the nasA gene and observed that the nasA 5'-coding sequence plays an important role in gene expression, as demonstrated by the fact that deletions caused over sixfold reduction in the expression of the lacZ reporter gene. Further analysis suggests that the nasA 5'-coding sequence promotes gene expression in a way that is not associated with weakened transcript folding around the translational initiation region or codon usage bias. The findings from this study imply that there exists potential to improve gene expression in A. vinelandii by optimizing 5'-coding sequences." @default.
• W2111625089 created "2016-06-24" @default.
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• W2111625089 creator A5043513976 @default.
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• W2111625089 date "2014-09-05" @default.
• W2111625089 modified "2023-09-27" @default.
• W2111625089 title "5′-coding sequence of thenasAgene ofAzotobacter vinelandiiis required for efficient expression" @default.
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• W2111625089 doi "https://doi.org/10.1111/1574-6968.12570" @default.
• W2111625089 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25110215" @default.
• W2111625089 hasPublicationYear "2014" @default.
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