Matches in SemOpenAlex for { <https://semopenalex.org/work/W2111670168> ?p ?o ?g. }
- W2111670168 endingPage "8" @default.
- W2111670168 startingPage "1450" @default.
- W2111670168 abstract "SR 48692, a selective nonpeptide antagonist of neurotensin (NT) receptors was developed recently. In the present work we studied the binding properties of the corresponding radioligand, [3H]SR 48692, in the adult guinea pig brain. The characterization of [3H]SR 48692 binding was carried out on brain membrane preparations and the distribution of [3H]SR 48692 binding sites was determined by receptor autoradiography and compared to that of [125I]NT binding sites. In brain homogenates, [3H]SR 48692 bound to a single population of sites with a Kd of 2.19 nM and a maximal binding capacity of 1.15 pmol/mg of protein. This maximal binding capacity value was 20 times higher than that observed for [125I]NT. NT agonists were able to interact competitively with the entire population of binding sites labeled by [3H]SR 48692, but their affinities were much lower than those observed for [125I]NT. By contrast, NT antagonists exhibited similar abilities to inhibit the binding of both radioligands. The addition of unlabeled NT in saturation assays revealed a competitive inhibition of [3H]SR 48692 binding, suggesting that agonist and antagonist ligand bind to overlapping domains of the NT receptor. The autoradiographic distribution of the low-affinity NT binding sites detected by [3H]SR 48692 (96% of the receptors) was very similar to the distribution of high-affinity receptors labeled with [125I]NT (4% of the receptors). In addition, the binding of [3H]SR 48692 was insensitive to guanyl nucleotides. Taken together, these findings suggest that the binding sites detected by [3H]SR 48692 in the guinea pig brain mainly represent the uncoupled form of the NT receptor." @default.
- W2111670168 created "2016-06-24" @default.
- W2111670168 creator A5010860742 @default.
- W2111670168 creator A5067919629 @default.
- W2111670168 creator A5070820842 @default.
- W2111670168 creator A5077150006 @default.
- W2111670168 creator A5078043271 @default.
- W2111670168 creator A5088092447 @default.
- W2111670168 date "1995-06-01" @default.
- W2111670168 modified "2023-09-27" @default.
- W2111670168 title "Characterization and distribution of binding sites for a new neurotensin receptor antagonist ligand, [3H]SR 48692, in the guinea pig brain." @default.
- W2111670168 cites W10911319 @default.
- W2111670168 cites W1543273725 @default.
- W2111670168 cites W159198934 @default.
- W2111670168 cites W1603624051 @default.
- W2111670168 cites W1607957872 @default.
- W2111670168 cites W1805549820 @default.
- W2111670168 cites W1861795153 @default.
- W2111670168 cites W1938887263 @default.
- W2111670168 cites W1972606382 @default.
- W2111670168 cites W1972948684 @default.
- W2111670168 cites W1976116089 @default.
- W2111670168 cites W1988226656 @default.
- W2111670168 cites W1995194650 @default.
- W2111670168 cites W1997228470 @default.
- W2111670168 cites W1997839253 @default.
- W2111670168 cites W2007941990 @default.
- W2111670168 cites W2016058820 @default.
- W2111670168 cites W2025549352 @default.
- W2111670168 cites W2033990363 @default.
- W2111670168 cites W2035807640 @default.
- W2111670168 cites W2041110976 @default.
- W2111670168 cites W2043813981 @default.
- W2111670168 cites W2044534393 @default.
- W2111670168 cites W2046207846 @default.
- W2111670168 cites W2052924733 @default.
- W2111670168 cites W2055438737 @default.
- W2111670168 cites W2059387399 @default.
- W2111670168 cites W2061640248 @default.
- W2111670168 cites W2062034250 @default.
- W2111670168 cites W2062912306 @default.
- W2111670168 cites W2068196787 @default.
- W2111670168 cites W2070020792 @default.
- W2111670168 cites W2073384618 @default.
- W2111670168 cites W2074631079 @default.
- W2111670168 cites W2076291793 @default.
- W2111670168 cites W2081610407 @default.
- W2111670168 cites W2086502238 @default.
- W2111670168 cites W2159939112 @default.
- W2111670168 cites W2254465644 @default.
- W2111670168 cites W2411560497 @default.
- W2111670168 cites W2424586145 @default.
- W2111670168 cites W2466399417 @default.
- W2111670168 cites W4298863787 @default.
- W2111670168 cites W1983876343 @default.
- W2111670168 cites W2346314362 @default.
- W2111670168 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4826444" @default.
- W2111670168 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7791120" @default.
- W2111670168 hasPublicationYear "1995" @default.
- W2111670168 type Work @default.
- W2111670168 sameAs 2111670168 @default.
- W2111670168 citedByCount "5" @default.
- W2111670168 countsByYear W21116701682014 @default.
- W2111670168 crossrefType "journal-article" @default.
- W2111670168 hasAuthorship W2111670168A5010860742 @default.
- W2111670168 hasAuthorship W2111670168A5067919629 @default.
- W2111670168 hasAuthorship W2111670168A5070820842 @default.
- W2111670168 hasAuthorship W2111670168A5077150006 @default.
- W2111670168 hasAuthorship W2111670168A5078043271 @default.
- W2111670168 hasAuthorship W2111670168A5088092447 @default.
- W2111670168 hasConcept C107824862 @default.
- W2111670168 hasConcept C116569031 @default.
- W2111670168 hasConcept C118303440 @default.
- W2111670168 hasConcept C12554922 @default.
- W2111670168 hasConcept C144024400 @default.
- W2111670168 hasConcept C149923435 @default.
- W2111670168 hasConcept C170493617 @default.
- W2111670168 hasConcept C185592680 @default.
- W2111670168 hasConcept C2776885963 @default.
- W2111670168 hasConcept C2777307743 @default.
- W2111670168 hasConcept C2778938600 @default.
- W2111670168 hasConcept C2778976297 @default.
- W2111670168 hasConcept C2908647359 @default.
- W2111670168 hasConcept C2909005528 @default.
- W2111670168 hasConcept C55493867 @default.
- W2111670168 hasConcept C67847695 @default.
- W2111670168 hasConcept C71240020 @default.
- W2111670168 hasConcept C86803240 @default.
- W2111670168 hasConceptScore W2111670168C107824862 @default.
- W2111670168 hasConceptScore W2111670168C116569031 @default.
- W2111670168 hasConceptScore W2111670168C118303440 @default.
- W2111670168 hasConceptScore W2111670168C12554922 @default.
- W2111670168 hasConceptScore W2111670168C144024400 @default.
- W2111670168 hasConceptScore W2111670168C149923435 @default.
- W2111670168 hasConceptScore W2111670168C170493617 @default.
- W2111670168 hasConceptScore W2111670168C185592680 @default.
- W2111670168 hasConceptScore W2111670168C2776885963 @default.
- W2111670168 hasConceptScore W2111670168C2777307743 @default.