FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2111681808> ?p ?o ?g. }

• W2111681808 endingPage "207" @default.
• W2111681808 startingPage "197" @default.
• W2111681808 abstract "Protein tyrosine phosphatases (PTPs) contain an essential thiol in the active site which may be susceptible to attack by nitric oxide-derived biological oxidants. We assessed the effects of peroxynitrite, nitric oxide, and S-nitrosoglutathione on the activity of three human tyrosine phosphatases in vitro. The receptor-like T-cell tyrosine phosphatase (CD45), the non-receptor-like tyrosine phosphatase PTP1B, and leukocyte-antigen-related (LAR) phosphatase were all irreversibly inactivated by peroxynitrite in less than 1 s with IC(50) values of </=0.9 microM. PTP inactivation was also seen with equivalent concentrations of peroxynitrite generated by SIN-1, indicating that bolus peroxynitrite and cogeneration of superoxide and nitric oxide were equipotent. Rate constants for peroxynitrite-mediated PTP inactivation were determined by competition with cysteine and were among the fastest rates yet seen for reaction of peroxynitrite with any biological molecules. The bimolecular reaction rates for CD45, LAR, and PTP1B were 2.0 x 10(8), 2.3 x 10(7), and 2.2 x 10(7) M(-1) s(-1), respectively. Inactivation by peroxynitrite was essentially irreversible as incubation with dithiothreitol (DTT) restored less than 10% of the original phosphatase activity. Prolonged treatment with 0.4 mM DETA NONOate, which generated a steady-state concentration of 2 microM nitric oxide, was only slightly inhibitory. S-Nitrosoglutathione (1.0 mM) inhibited PTPs by approximately 50% after 30 min and the inhibition was completely reversed by DTT. Nitrotyrosine immunoblots of peroxynitrite-treated PTP1B revealed that peroxynitrite completely inactivated PTP1B prior to the appearance of protein tyrosine nitration. Peroxynitrite anion is structurally similar to phosphate anion both in terms of molecular diameter and charge. Thus, the extreme vulnerability of these PTPs to peroxynitrite-mediated inactivation is consistent with attraction of peroxynitrite anion to the active site and subsequent oxidation of the essential thiolate. These findings suggest that any PTP possessing the CXXXXXR active-site sequence could potentially be inactivated by peroxynitrite in vivo resulting in a net increase in tyrosine phosphorylation and profound effects on phosphotyrosine-dependent signaling cascades." @default.
• W2111681808 created "2016-06-24" @default.
• W2111681808 creator A5010895814 @default.
• W2111681808 creator A5021164517 @default.
• W2111681808 creator A5044791769 @default.
• W2111681808 creator A5047777156 @default.
• W2111681808 date "1999-09-01" @default.
• W2111681808 modified "2023-10-18" @default.
• W2111681808 title "Rapid and Irreversible Inactivation of Protein Tyrosine Phosphatases PTP1B, CD45, and LAR by Peroxynitrite" @default.
• W2111681808 cites W136427227 @default.
• W2111681808 cites W1500094754 @default.
• W2111681808 cites W1505685265 @default.
• W2111681808 cites W1511153178 @default.
• W2111681808 cites W1527362740 @default.
• W2111681808 cites W1535816350 @default.
• W2111681808 cites W1553375567 @default.
• W2111681808 cites W1760998103 @default.
• W2111681808 cites W1761291408 @default.
• W2111681808 cites W179980497 @default.
• W2111681808 cites W1821918060 @default.
• W2111681808 cites W1829813323 @default.
• W2111681808 cites W1889110369 @default.
• W2111681808 cites W1923609106 @default.
• W2111681808 cites W1967433712 @default.
• W2111681808 cites W1969941249 @default.
• W2111681808 cites W1970184469 @default.
• W2111681808 cites W1978679885 @default.
• W2111681808 cites W1982422614 @default.
• W2111681808 cites W1982778052 @default.
• W2111681808 cites W1983102597 @default.
• W2111681808 cites W1984001765 @default.
• W2111681808 cites W1987861061 @default.
• W2111681808 cites W1990974797 @default.
• W2111681808 cites W1997044687 @default.
• W2111681808 cites W2001413102 @default.
• W2111681808 cites W2004356154 @default.
• W2111681808 cites W2009958807 @default.
• W2111681808 cites W2011582460 @default.
• W2111681808 cites W2011948827 @default.
• W2111681808 cites W2012514869 @default.
• W2111681808 cites W2022377100 @default.
• W2111681808 cites W2022786966 @default.
• W2111681808 cites W2023632622 @default.
• W2111681808 cites W2027641362 @default.
• W2111681808 cites W2029931901 @default.
• W2111681808 cites W2032618552 @default.
• W2111681808 cites W2035743979 @default.
• W2111681808 cites W2035921541 @default.
• W2111681808 cites W2039626545 @default.
• W2111681808 cites W2046798893 @default.
• W2111681808 cites W2050157380 @default.
• W2111681808 cites W2050465795 @default.
• W2111681808 cites W2056135909 @default.
• W2111681808 cites W2058013605 @default.
• W2111681808 cites W2058137293 @default.
• W2111681808 cites W2067753981 @default.
• W2111681808 cites W2074838372 @default.
• W2111681808 cites W2085664820 @default.
• W2111681808 cites W2087335054 @default.
• W2111681808 cites W2088072401 @default.
• W2111681808 cites W2090316954 @default.
• W2111681808 cites W2091709735 @default.
• W2111681808 cites W2093708814 @default.
• W2111681808 cites W2093987948 @default.
• W2111681808 cites W2094213529 @default.
• W2111681808 cites W2094238895 @default.
• W2111681808 cites W2094378349 @default.
• W2111681808 cites W2109654316 @default.
• W2111681808 cites W2118912669 @default.
• W2111681808 cites W2136573391 @default.
• W2111681808 cites W2144436378 @default.
• W2111681808 cites W2160421139 @default.
• W2111681808 cites W2228279685 @default.
• W2111681808 cites W4248673749 @default.
• W2111681808 doi "https://doi.org/10.1006/abbi.1999.1374" @default.
• W2111681808 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10486138" @default.
• W2111681808 hasPublicationYear "1999" @default.
• W2111681808 type Work @default.
• W2111681808 sameAs 2111681808 @default.
• W2111681808 citedByCount "187" @default.
• W2111681808 countsByYear W21116818082012 @default.
• W2111681808 countsByYear W21116818082013 @default.
• W2111681808 countsByYear W21116818082014 @default.
• W2111681808 countsByYear W21116818082015 @default.
• W2111681808 countsByYear W21116818082016 @default.
• W2111681808 countsByYear W21116818082017 @default.
• W2111681808 countsByYear W21116818082018 @default.
• W2111681808 countsByYear W21116818082019 @default.
• W2111681808 countsByYear W21116818082020 @default.
• W2111681808 countsByYear W21116818082022 @default.
• W2111681808 countsByYear W21116818082023 @default.
• W2111681808 crossrefType "journal-article" @default.
• W2111681808 hasAuthorship W2111681808A5010895814 @default.
• W2111681808 hasAuthorship W2111681808A5021164517 @default.
• W2111681808 hasAuthorship W2111681808A5044791769 @default.
• W2111681808 hasAuthorship W2111681808A5047777156 @default.
• W2111681808 hasConcept C178666793 @default.
• W2111681808 hasConcept C178790620 @default.

• next