FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2111686506> ?p ?o ?g. }

• W2111686506 endingPage "276" @default.
• W2111686506 startingPage "262" @default.
• W2111686506 abstract "The repressor proteins of the LacI/GalR family exhibit significant similarity in their secondary and tertiary structures despite less than 35% identity in their primary sequences. Furthermore, the core domains of these oligomeric repressors, which mediate dimerization, are homologous with the monomeric periplasmic binding proteins, extending the issue of plasticity to quaternary structure. To elucidate the determinants of assembly, a structure-based alignment has been created for three repressors and four periplasmic binding proteins. Contact maps have also been constructed for the three repressor interfaces to distinguish any conserved interactions. These analyses show few strict requirements for assembly of the core N-subdomain interface. The interfaces of repressor core C-subdomains are well conserved at the structural level, and their primary sequences differ significantly from the monomeric periplasmic binding proteins at positions equivalent to LacI 281 and 282. However, previous biochemical and phenotypic analyses indicate that LacI tolerates many mutations at 281. Mutations at LacI 282 were shown to abrogate assembly, but for Y282D this could be compensated by a second-site mutation in the core N-subdomain at K84 to L or A. Using the link between LacI assembly and function, we have further identified 22 second-site mutations that compensate the Y282D dimerization defect in vivo. The sites of these mutations fall into several structural regions, each of which may influence assembly by a different mechanism. Thus, the 360-amino acid scaffold of LacI allows plasticity of its quaternary structure. The periplasmic binding proteins may require only minimal changes to facilitate oligomerization similar to the repressor proteins." @default.
• W2111686506 created "2016-06-24" @default.
• W2111686506 creator A5035852381 @default.
• W2111686506 date "2001-02-01" @default.
• W2111686506 modified "2023-10-16" @default.
• W2111686506 title "Plasticity of quaternary structure: Twenty-two ways to form a LacI dimer" @default.
• W2111686506 cites W1481557920 @default.
• W2111686506 cites W1497443366 @default.
• W2111686506 cites W1499816395 @default.
• W2111686506 cites W1511055887 @default.
• W2111686506 cites W1523367337 @default.
• W2111686506 cites W1528121124 @default.
• W2111686506 cites W1531576924 @default.
• W2111686506 cites W1550088392 @default.
• W2111686506 cites W1551143570 @default.
• W2111686506 cites W1567146791 @default.
• W2111686506 cites W1576231449 @default.
• W2111686506 cites W1576465448 @default.
• W2111686506 cites W1578410723 @default.
• W2111686506 cites W1636701701 @default.
• W2111686506 cites W1857623540 @default.
• W2111686506 cites W1965092356 @default.
• W2111686506 cites W1973481676 @default.
• W2111686506 cites W1980644102 @default.
• W2111686506 cites W1984874903 @default.
• W2111686506 cites W1987063642 @default.
• W2111686506 cites W1999503252 @default.
• W2111686506 cites W2004576601 @default.
• W2111686506 cites W2006944230 @default.
• W2111686506 cites W2009217296 @default.
• W2111686506 cites W2010260387 @default.
• W2111686506 cites W2013127795 @default.
• W2111686506 cites W2013596926 @default.
• W2111686506 cites W2014201888 @default.
• W2111686506 cites W2023790301 @default.
• W2111686506 cites W2039626541 @default.
• W2111686506 cites W2040407809 @default.
• W2111686506 cites W2040423444 @default.
• W2111686506 cites W2051010979 @default.
• W2111686506 cites W2053766516 @default.
• W2111686506 cites W2055043387 @default.
• W2111686506 cites W2055233956 @default.
• W2111686506 cites W2063492240 @default.
• W2111686506 cites W2064304947 @default.
• W2111686506 cites W2066825888 @default.
• W2111686506 cites W2072920810 @default.
• W2111686506 cites W2075598295 @default.
• W2111686506 cites W2083060817 @default.
• W2111686506 cites W2090432482 @default.
• W2111686506 cites W2091295803 @default.
• W2111686506 cites W2094086949 @default.
• W2111686506 cites W2094313036 @default.
• W2111686506 cites W2114128178 @default.
• W2111686506 cites W2133444627 @default.
• W2111686506 cites W2134328540 @default.
• W2111686506 cites W2156440952 @default.
• W2111686506 cites W2157593554 @default.
• W2111686506 cites W2161653032 @default.
• W2111686506 cites W2398547371 @default.
• W2111686506 cites W4245223836 @default.
• W2111686506 doi "https://doi.org/10.1110/ps.35801" @default.
• W2111686506 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2373939" @default.
• W2111686506 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11266612" @default.
• W2111686506 hasPublicationYear "2001" @default.
• W2111686506 type Work @default.
• W2111686506 sameAs 2111686506 @default.
• W2111686506 citedByCount "41" @default.
• W2111686506 countsByYear W21116865062013 @default.
• W2111686506 countsByYear W21116865062014 @default.
• W2111686506 countsByYear W21116865062015 @default.
• W2111686506 countsByYear W21116865062016 @default.
• W2111686506 countsByYear W21116865062017 @default.
• W2111686506 countsByYear W21116865062018 @default.
• W2111686506 countsByYear W21116865062019 @default.
• W2111686506 countsByYear W21116865062020 @default.
• W2111686506 countsByYear W21116865062021 @default.
• W2111686506 countsByYear W21116865062022 @default.
• W2111686506 countsByYear W21116865062023 @default.
• W2111686506 crossrefType "journal-article" @default.
• W2111686506 hasAuthorship W2111686506A5035852381 @default.
• W2111686506 hasBestOaLocation W21116865062 @default.
• W2111686506 hasConcept C104292427 @default.
• W2111686506 hasConcept C104317684 @default.
• W2111686506 hasConcept C107824862 @default.
• W2111686506 hasConcept C113629113 @default.
• W2111686506 hasConcept C12554922 @default.
• W2111686506 hasConcept C158448853 @default.
• W2111686506 hasConcept C185592680 @default.
• W2111686506 hasConcept C201663137 @default.
• W2111686506 hasConcept C47701112 @default.
• W2111686506 hasConcept C54355233 @default.
• W2111686506 hasConcept C547475151 @default.
• W2111686506 hasConcept C55493867 @default.
• W2111686506 hasConcept C7927669 @default.
• W2111686506 hasConcept C86339819 @default.
• W2111686506 hasConcept C86803240 @default.
• W2111686506 hasConceptScore W2111686506C104292427 @default.
• W2111686506 hasConceptScore W2111686506C104317684 @default.

• next