Matches in SemOpenAlex for { <https://semopenalex.org/work/W2111729216> ?p ?o ?g. }
- W2111729216 endingPage "e1001066" @default.
- W2111729216 startingPage "e1001066" @default.
- W2111729216 abstract "In spite of decades-long studies, the mechanism of morphogenesis of plus-stranded RNA viruses belonging to the genus Enterovirus of Picornaviridae, including poliovirus (PV), is not understood. Numerous attempts to identify an RNA encapsidation signal have failed. Genetic studies, however, have implicated a role of the non-structural protein 2CATPase in the formation of poliovirus particles. Here we report a novel mechanism in which protein-protein interaction is sufficient to explain the specificity in PV encapsidation. Making use of a novel “reporter virus”, we show that a quasi-infectious chimera consisting of the capsid precursor of C-cluster coxsackie virus 20 (C-CAV20) and the nonstructural proteins of the closely related PV translated and replicated its genome with wild type kinetics, whereas encapsidation was blocked. On blind passages, encapsidation of the chimera was rescued by a single mutation either in capsid protein VP3 of CAV20 or in 2CATPase of PV. Whereas each of the single-mutation variants expressed severe proliferation phenotypes, engineering both mutations into the chimera yielded a virus encapsidating with wild type kinetics. Biochemical analyses provided strong evidence for a direct interaction between 2CATPase and VP3 of PV and CAV20. Chimeras of other C-CAVs (CAV20/CAV21 or CAV18/CAV20) were blocked in encapsidation (no virus after blind passages) but could be rescued if the capsid and 2CATPase coding regions originated from the same virus. Our novel mechanism explains the specificity of encapsidation without apparent involvement of an RNA signal by considering that (i) genome replication is known to be stringently linked to translation, (ii) morphogenesis is known to be stringently linked to genome replication, (iii) newly synthesized 2CATPase is an essential component of the replication complex, and (iv) 2CATPase has specific affinity to capsid protein(s). These conditions lead to morphogenesis at the site where newly synthesized genomes emerge from the replication complex." @default.
- W2111729216 created "2016-06-24" @default.
- W2111729216 creator A5005602487 @default.
- W2111729216 creator A5022355218 @default.
- W2111729216 creator A5030376340 @default.
- W2111729216 creator A5034083570 @default.
- W2111729216 creator A5052633582 @default.
- W2111729216 creator A5073909769 @default.
- W2111729216 date "2010-08-26" @default.
- W2111729216 modified "2023-10-10" @default.
- W2111729216 title "Direct Interaction between Two Viral Proteins, the Nonstructural Protein 2CATPase and the Capsid Protein VP3, Is Required for Enterovirus Morphogenesis" @default.
- W2111729216 cites W1483254706 @default.
- W2111729216 cites W1496743229 @default.
- W2111729216 cites W1541696172 @default.
- W2111729216 cites W1556270435 @default.
- W2111729216 cites W1580495826 @default.
- W2111729216 cites W1584025851 @default.
- W2111729216 cites W1595782547 @default.
- W2111729216 cites W1825835878 @default.
- W2111729216 cites W1840105038 @default.
- W2111729216 cites W1905288701 @default.
- W2111729216 cites W1914843804 @default.
- W2111729216 cites W1916164841 @default.
- W2111729216 cites W1922282369 @default.
- W2111729216 cites W1962051008 @default.
- W2111729216 cites W1965549451 @default.
- W2111729216 cites W1968128199 @default.
- W2111729216 cites W1972277908 @default.
- W2111729216 cites W1978512485 @default.
- W2111729216 cites W1985274927 @default.
- W2111729216 cites W1985638466 @default.
- W2111729216 cites W1986496785 @default.
- W2111729216 cites W1987977026 @default.
- W2111729216 cites W1990884146 @default.
- W2111729216 cites W2004121444 @default.
- W2111729216 cites W2009678288 @default.
- W2111729216 cites W2026369794 @default.
- W2111729216 cites W2027751877 @default.
- W2111729216 cites W2030137405 @default.
- W2111729216 cites W2032887139 @default.
- W2111729216 cites W2039370848 @default.
- W2111729216 cites W2043326986 @default.
- W2111729216 cites W2050524167 @default.
- W2111729216 cites W2050594447 @default.
- W2111729216 cites W2060520834 @default.
- W2111729216 cites W2065863039 @default.
- W2111729216 cites W2068353835 @default.
- W2111729216 cites W2072690167 @default.
- W2111729216 cites W2075557556 @default.
- W2111729216 cites W2081411566 @default.
- W2111729216 cites W2081913826 @default.
- W2111729216 cites W2082414564 @default.
- W2111729216 cites W2085942808 @default.
- W2111729216 cites W2089782688 @default.
- W2111729216 cites W2092019188 @default.
- W2111729216 cites W2097446370 @default.
- W2111729216 cites W2099323362 @default.
- W2111729216 cites W2099685714 @default.
- W2111729216 cites W2102253488 @default.
- W2111729216 cites W2102787346 @default.
- W2111729216 cites W2103705071 @default.
- W2111729216 cites W2106530317 @default.
- W2111729216 cites W2112955990 @default.
- W2111729216 cites W2113416147 @default.
- W2111729216 cites W2115846714 @default.
- W2111729216 cites W2116474159 @default.
- W2111729216 cites W2118244493 @default.
- W2111729216 cites W2118666572 @default.
- W2111729216 cites W2120768970 @default.
- W2111729216 cites W2121255902 @default.
- W2111729216 cites W2121646171 @default.
- W2111729216 cites W2124771674 @default.
- W2111729216 cites W2124901211 @default.
- W2111729216 cites W2127658975 @default.
- W2111729216 cites W2129795909 @default.
- W2111729216 cites W2133432444 @default.
- W2111729216 cites W2136843465 @default.
- W2111729216 cites W2141558821 @default.
- W2111729216 cites W2143830829 @default.
- W2111729216 cites W2144538210 @default.
- W2111729216 cites W2146894910 @default.
- W2111729216 cites W2148270513 @default.
- W2111729216 cites W2154408609 @default.
- W2111729216 cites W2171241405 @default.
- W2111729216 cites W2184154955 @default.
- W2111729216 cites W87599195 @default.
- W2111729216 doi "https://doi.org/10.1371/journal.ppat.1001066" @default.
- W2111729216 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2928791" @default.
- W2111729216 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20865167" @default.
- W2111729216 hasPublicationYear "2010" @default.
- W2111729216 type Work @default.
- W2111729216 sameAs 2111729216 @default.
- W2111729216 citedByCount "112" @default.
- W2111729216 countsByYear W21117292162012 @default.
- W2111729216 countsByYear W21117292162013 @default.
- W2111729216 countsByYear W21117292162014 @default.
- W2111729216 countsByYear W21117292162015 @default.
- W2111729216 countsByYear W21117292162016 @default.