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• W2111931465 abstract "ABSTRACT Enterocin AS-48 is a cyclic peptide produced by Enterococcus faecalis S-48 whose genetic determinants have been identified in the conjugative plasmid pMB2. A region of 7.8 kb, carrying the minimum information required for production of and immunity against AS-48, had been previously cloned and sequenced in pAM401 (pAM401-52). In this region, the as-48A structural gene and as-48B , as-48C , as-48C 1 , as-48D , and as-48D 1 genes and open reading frame 6 (ORF6) and ORF7 had been identified. The sequence analysis carried out in this work in the Bgl II B fragment (6.6-kb) from pMB2 cloned downstream from the last ORF identified (ORF7) revealed the existence of two new ORFs, as-48G and as-48H , necessary for full AS-48 expression. Thus, JH2-2 transformants obtained with the pAM401-81 plasmid became producers and resistant at the wild-type level. Tn 5 disruption experiments in the last genes, as-48EFGH , were not able to reproduce these expression levels, confirming that expression of these genes is necessary to get the phenotype conferred by the wild-type pMB2 plasmid. The as-48EFGH operon encodes a new ABC transporter that could be involved in producer self-protection. On the basis of the observed similarities, As-48G would be the ATP-binding domain, the deduced amino acid sequences of As-48E and As48-H could be assigned as transmembrane subunits, and As-48F, with an N-terminal transmembrane segment and a coiled-coil domain, strongly resembles the structure of some known ABC transporter accessory proteins whose localization in the cell is discussed. This cluster of genes is expressed by two polycistronic mRNAs, T 2 and T 3 , in JH2-2(pAM401-81) in coordinate expression. Our results also suggest that expression of T 3 could be regulated, because in JH2-2(pAM401 EH ) transformants, T 3 was not detected, suggesting that these genes do not by themselves confer immunity, in accordance with the requirement for the as-48D 1 gene for immunity against AS-48." @default.
• W2111931465 created "2016-06-24" @default.
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• W2111931465 date "2003-02-01" @default.
• W2111931465 modified "2023-10-16" @default.
• W2111931465 title "Characterization of a New Operon, <i>as-48EFGH</i> , from the <i>as-48</i> Gene Cluster Involved in Immunity to Enterocin AS-48" @default.
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• W2111931465 doi "https://doi.org/10.1128/aem.69.2.1229-1236.2003" @default.
• W2111931465 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/143590" @default.
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