FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2112008078> ?p ?o ?g. }

• W2112008078 endingPage "108" @default.
• W2112008078 startingPage "101" @default.
• W2112008078 abstract "Factors such as warm ischemia and time at room temperature before tissue treatment may influence the results of mRNA expression analyses on tissue specimens obtained during surgery. We evaluated the effect of these factors on RNA integrity and mRNA expression levels by incubating freshly obtained mouse liver tissue at 25 or 37 °C for periods of 0–4 h. Changes in the mRNA expression levels of seven genes, Tbp, Eef1a, Fos, Junb, Myc, Vegf, and Glut2, were determined by real-time reverse transcription-polymerase chain reaction. Incubation at 25 °C for up to 4 h only slightly altered (by a factor of less than 2) levels of mRNA for Tbp, Eef1a, Junb, Myc, Vegf, and Glut2. This result is consistent with limited RNA degradation at this temperature. Incubation at 37 °C strongly affected the levels of these mRNAs. Four hours of incubation at this temperature resulted in extensive RNA degradation, with mRNA levels falling to 1/10th those before incubation. When relative quantification was performed, i.e., quantification of the target gene transcripts in comparison to an endogenous housekeeping transcript (Tbp or Eef1a), the changes in mRNA levels were reduced to less than 2.5-fold. Fos behaved very differently from the other genes tested on incubation, with Fos mRNA levels increasing considerably following incubation at either 25 or 37 °C. Our data suggest that, with the exception of certain genes induced by tissue injury, relative quantification of mRNA, even on degraded RNA samples, can provide a reliable estimate of in vivo mRNA levels." @default.
• W2112008078 created "2016-06-24" @default.
• W2112008078 creator A5039283945 @default.
• W2112008078 creator A5046624926 @default.
• W2112008078 creator A5085271440 @default.
• W2112008078 creator A5088103291 @default.
• W2112008078 date "2004-05-01" @default.
• W2112008078 modified "2023-10-17" @default.
• W2112008078 title "Gene expression analysis by real-time reverse transcription polymerase chain reaction: influence of tissue handling" @default.
• W2112008078 cites W1601826601 @default.
• W2112008078 cites W1965854549 @default.
• W2112008078 cites W1970156673 @default.
• W2112008078 cites W1971964615 @default.
• W2112008078 cites W1987447923 @default.
• W2112008078 cites W2000977514 @default.
• W2112008078 cites W2003274334 @default.
• W2112008078 cites W2006874202 @default.
• W2112008078 cites W2015344488 @default.
• W2112008078 cites W2016613744 @default.
• W2112008078 cites W2016929067 @default.
• W2112008078 cites W2018013926 @default.
• W2112008078 cites W2021253208 @default.
• W2112008078 cites W2031843032 @default.
• W2112008078 cites W2042917826 @default.
• W2112008078 cites W2047443734 @default.
• W2112008078 cites W2047488088 @default.
• W2112008078 cites W2057460273 @default.
• W2112008078 cites W2061348306 @default.
• W2112008078 cites W2062798136 @default.
• W2112008078 cites W2077458979 @default.
• W2112008078 cites W2083440368 @default.
• W2112008078 cites W2091325747 @default.
• W2112008078 cites W2097255042 @default.
• W2112008078 cites W2108244474 @default.
• W2112008078 cites W2140557689 @default.
• W2112008078 cites W2143146123 @default.
• W2112008078 cites W2145684092 @default.
• W2112008078 cites W2146264532 @default.
• W2112008078 cites W2164515753 @default.
• W2112008078 cites W2164578725 @default.
• W2112008078 cites W2314374552 @default.
• W2112008078 cites W4213387068 @default.
• W2112008078 cites W4235034935 @default.
• W2112008078 doi "https://doi.org/10.1016/j.ab.2004.02.004" @default.
• W2112008078 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15113684" @default.
• W2112008078 hasPublicationYear "2004" @default.
• W2112008078 type Work @default.
• W2112008078 sameAs 2112008078 @default.
• W2112008078 citedByCount "79" @default.
• W2112008078 countsByYear W21120080782012 @default.
• W2112008078 countsByYear W21120080782013 @default.
• W2112008078 countsByYear W21120080782014 @default.
• W2112008078 countsByYear W21120080782015 @default.
• W2112008078 countsByYear W21120080782016 @default.
• W2112008078 countsByYear W21120080782017 @default.
• W2112008078 countsByYear W21120080782018 @default.
• W2112008078 countsByYear W21120080782019 @default.
• W2112008078 countsByYear W21120080782020 @default.
• W2112008078 countsByYear W21120080782021 @default.
• W2112008078 countsByYear W21120080782022 @default.
• W2112008078 crossrefType "journal-article" @default.
• W2112008078 hasAuthorship W2112008078A5039283945 @default.
• W2112008078 hasAuthorship W2112008078A5046624926 @default.
• W2112008078 hasAuthorship W2112008078A5085271440 @default.
• W2112008078 hasAuthorship W2112008078A5088103291 @default.
• W2112008078 hasConcept C104317684 @default.
• W2112008078 hasConcept C105580179 @default.
• W2112008078 hasConcept C150194340 @default.
• W2112008078 hasConcept C153911025 @default.
• W2112008078 hasConcept C25642318 @default.
• W2112008078 hasConcept C2781085546 @default.
• W2112008078 hasConcept C33922130 @default.
• W2112008078 hasConcept C4692481 @default.
• W2112008078 hasConcept C48023723 @default.
• W2112008078 hasConcept C55493867 @default.
• W2112008078 hasConcept C67705224 @default.
• W2112008078 hasConcept C86803240 @default.
• W2112008078 hasConceptScore W2112008078C104317684 @default.
• W2112008078 hasConceptScore W2112008078C105580179 @default.
• W2112008078 hasConceptScore W2112008078C150194340 @default.
• W2112008078 hasConceptScore W2112008078C153911025 @default.
• W2112008078 hasConceptScore W2112008078C25642318 @default.
• W2112008078 hasConceptScore W2112008078C2781085546 @default.
• W2112008078 hasConceptScore W2112008078C33922130 @default.
• W2112008078 hasConceptScore W2112008078C4692481 @default.
• W2112008078 hasConceptScore W2112008078C48023723 @default.
• W2112008078 hasConceptScore W2112008078C55493867 @default.
• W2112008078 hasConceptScore W2112008078C67705224 @default.
• W2112008078 hasConceptScore W2112008078C86803240 @default.
• W2112008078 hasIssue "2" @default.
• W2112008078 hasLocation W21120080781 @default.
• W2112008078 hasLocation W21120080782 @default.
• W2112008078 hasOpenAccess W2112008078 @default.
• W2112008078 hasPrimaryLocation W21120080781 @default.
• W2112008078 hasRelatedWork W1512954123 @default.
• W2112008078 hasRelatedWork W2043338644 @default.
• W2112008078 hasRelatedWork W2059288109 @default.
• W2112008078 hasRelatedWork W2060751813 @default.

• next